A laser fluorimeter for direct cardiac metabolism investigation
A laser fluorimeter for direct cardiac metabolism investigation
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DOI：
10.1016/0030-3992(82)90110-4
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发表时间：
1982-06
影响因子：
5
通讯作者：
G. Renault;E. Raynal;M. Sinet;J. Berthier;B. Godard;J. Cornillault
中科院分区：
文献类型：
--
作者：
G. Renault;E. Raynal;M. Sinet;J. Berthier;B. Godard;J. Cornillault
我们开发了一种装置，包括两个激光源（氮气和染料激光器）、一个光学系统、一根能够传输紫外光和红外光的光纤、光接收器和一个为微型计算机供电的模拟电路，以便通过荧光测定法对 NADH/NAD 比率进行原位在线研究。本文包括该方法的科学基础、设备各部分的特点以及在研究心脏代谢或任何可通过荧光法到达的器官中的可能应用。导管插入术、纤维镜检查或简单的经皮穿刺。提供了为了解缺氧或缺血对组织 NADH 水平的影响而进行的实验示例。
We have developed a device which includes two laser sources (nitrogen and dye lasers), an optical system, an optical fibre able to transmit both ultra-violet and infra-red light, photoreceivers, and an analogue circuit feeding a microcomputer in order to perform anin situand on line study of NADH/NAD ratios by fluorimetry.The paper includes the scientific basis of the method, the characteristics of each part of the apparatus and possible applications to the study of cardiac metabolism or any organ that can be reached through catheterism, fibrescopy or simple transcutaneous puncture. Examples of experiments carried out to appreciate the effects of anoxia or ischemia on the tissular NADH level are presented.