Fetal membranes exhibit selective leukocyte chemotaxic activity during human labor
Fetal membranes exhibit selective leukocyte chemotaxic activity during human labor
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DOI：
10.1016/j.jri.2009.01.002
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发表时间：
2009-06-01
影响因子：
3.4
通讯作者：
Vadillo-Ortega, R.
中科院分区：
文献类型：
--
作者：
Gomez-Lopez, N.;Estrada-Gutierrez, G.;Vadillo-Ortega, R.
女性分娩过程的特征之一是白细胞进入生殖组织。这些迁移细胞可能在诱导与分娩过程中胎膜破裂相关的功能和生化变化中发挥作用。本研究的目的是评估人胎膜是否在分娩过程中诱导白细胞趋化性，以及鉴定和表征这些组织分泌的白细胞趋化因子。将足月妊娠和自发活跃分娩妇女的胎膜提取物的白细胞趋化活性与未分娩妇女的提取物进行比较。用流式细胞仪分析白细胞的数量和表型。使用多重系统定量趋化因子，并通过免疫荧光组织化学进行鉴定。虽然所有测试的提取物诱导白细胞的趋化性，那些准备从妇女分娩诱导更高的反应。多形核白细胞趋化性增加约三倍，以响应提取物从胎膜与劳动。与未分娩妇女的提取物相比，相同的提取物引起所吸引的单核细胞（36倍）以及T和B淋巴细胞和NK细胞（均为5倍）显著增加。这种增强的趋化活性与IL-8、MCP-1、UP-10和MIP-1 α的存在有关。我们的结论是，胎膜提取物从妇女在分娩过程中表现出特定的白细胞亚群在体外的选择性趋化。这个过程可能有助于由特定白细胞组成的微环境，促进和放大分娩期间胎膜中的生化变化。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
One of the characteristics of the labor process in women is leukocyte recruitment into reproductive tissues. These migrating cells may play a role in the induction of functional and biochemical changes associated with the rupture of fetal membranes during labor. This study was undertaken to assess whether human fetal membranes induce leukocyte chemotaxis during labor as well as to identify and characterize leukocyte chemoattractants secreted by these tissues. Leukocyte chemotactic activity of fetal membrane extracts obtained from women with full-term pregnancies and spontaneous active labor was compared with extracts from women without labor. The number and phenotype of attracted leukocytes were analyzed by flow cytometry. Chemokines were quantified using a Multiplex system and were identified by immunofluorescence histochemistry. Although all tested extracts induced chemotaxis of leukocytes, those prepared from women undergoing labor induced higher responses. Polymorphonuclear leukocyte chemotaxis increased approximately three-fold in response to extract from fetal membranes with labor. The same extracts elicited a significant increase in attracted monocytes (36-fold) as well as T and B lymphocytes, and NK cells (all five-fold) when compared to extracts from women without labor. This enhanced chemotactic activity was associated with the presence of IL-8, MCP-1, UP-10 and MIP-1 alpha. We conclude that fetal membrane extracts obtained from women during labor exhibit selective chemotaxis for specific leukocyte subpopulations in vitro. This process may contribute to a microenvironment composed of specific leukocytes that promotes and amplifies biochemical changes in the fetal membranes during labor. (C) 2009 Elsevier Ireland Ltd. All rights reserved.