TEMPORAL AND SPATIAL EXPRESSIONS OF TYPE-XII COLLAGEN IN THE REMODELING PERIODONTAL-LIGAMENT DURING EXPERIMENTAL TOOTH MOVEMENT
TEMPORAL AND SPATIAL EXPRESSIONS OF TYPE-XII COLLAGEN IN THE REMODELING PERIODONTAL-LIGAMENT DURING EXPERIMENTAL TOOTH MOVEMENT
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DOI：
10.1177/00220345950740010501
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发表时间：
1995-01-01
影响因子：
7.6
通讯作者：
NISHIMURA, I
中科院分区：
文献类型：
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作者：
KARIMBUX, NY;NISHIMURA, I
本研究验证了一个假设，即成人牙周膜（PDL）的重建过程中重申的细胞和分子的事件发生在发展过程中顺序。XII型胶原蛋白与PDL细胞外基质的三维组织有关，其表达仅限于终末分化阶段。本研究的重点是在大鼠实验性PDL重塑过程中XII型胶原的时空表达的检查。I型和XII型胶原mRNA的时间表达分别通过RNA转移印迹和RNA酶保护试验进行了检查，并发现在整个实验期间对照组相对稳定。在牙齿移动组中，I型胶原蛋白的表达在72小时增加，并在一周内维持高水平的表达，而XII型胶原蛋白的表达在一周内首次出现增加。I型和XII型胶原蛋白的表达在重塑中的时间激活发生在类似于PDL发展过程中发现的模式中。原位杂交检测牙齿移动一周后标本中XII型胶原mRNA的空间表达。标记的细胞，这是更明显的张力侧，通常表现出梭形，并包围着成熟的PDL矩阵。我们的数据表明，十二型胶原的表达可能与PDL的功能性再生密切相关。
This study tested the hypothesis that the remodeling processes of adult periodontal ligament (PDL) reiterate the cellular and molecular events that occur sequentially during development. Type XII collagen has been implicated in the three-dimensional organization of the PDL extracellular matrix, and its expression has been restricted to the terminally differentiated stages. This study focused on the examination of the temporal and spatial expression of type XII collagen during experimental PDL remodeling in the rat. The temporal expressions of types I and XII collagen mRNAs were examined by RNA transfer blot and RNase protection assays, respectively, and were found to be relatively stable in the control group throughout the experimental period. In the tooth movement group, the expression of type I collagen increased at 72 hours and sustained the high level of expression at one week, while an increase in the expression of type XII collagen was first noted at the one-week period. The temporal activation of types I and XII collagen expression in the remodeling occurred in a pattern similar to that found during the development of the PDL. The spatial expression of type XII collagen mRNA was examined by in situ hybridization in the one-week-tooth-movement specimens. Labeled cells, which were more evident in the tension side, typically exhibited a spindle shape and were surrounded by the mature PDL matrix. Our data suggest that the type XII collagen expression may be closely associated with the functional regeneration of the PDL.