Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge
Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge
复制标题
DOI：
10.1016/j.cbpb.2004.04.005
复制
发表时间：
2004-07-01
影响因子：
2.3
通讯作者：
Lee, TH
中科院分区：
文献类型：
--
作者：
Lin, CH;Tsai, RS;Lee, TH
淡水(FW)斑点绿色河豚(Tetraodon Nigroviridis)直接从当地水族馆转移到实验室的淡水(FW；千分之0)、微咸水(BW；千分之15)和海水(SW；千分之35)条件下，饲养至少两周。未发现死亡病例。为探讨真盐性硬骨鱼渗透调节的有效机制，研究了Na，K-ATPase(NKA)在河豚鳃和肾中的分布和表达，并测定了血液中的渗透压、[Na+]和[Cl-]。BW组的相对蛋白丰度和活性最低，而SW组的相对蛋白丰度和活性高于FW组。在所有盐度中，鳃NKA免疫反应仅见于纤丝板层间区的上皮细胞，而在板层上未见。FW河豚肾脏NKAα亚基的相对丰度和NKA活性均高于BW和SW。在不同盐度的鱼群中，肾NKA存在于远端小管、近端小管和集合管的上皮细胞中，而不存在于肾小球。FW河豚的血浆渗透压和氯离子水平显著低于BW和SW，而血钠水平在各组之间没有差异。尽管在FW、BW或SW驯化的斑点绿河豚的鳃和肾脏中发现了相同的NKA分布，但不同盐度组的鱼NKA表达的差异与生理稳态(稳定的血液渗透压)有关，并表明这种淡水和河口物种对盐度挑战具有令人印象深刻的渗透调节能力。(C)2004 Elsevier Inc.保留所有权利。
Freshwater (FW) spotted green pufferfish (Tetraodon nigroviridis) were transferred directly from a local aquarium to fresh water (FW; 0parts per thousand), brackish water (BW; 15parts per thousand), and seawater (SW; 35parts per thousand) conditions in the laboratory and reared for at least two weeks. No mortality was found. To investigate the efficient mechanisms of osmoregulation in the euryhaline teleost, distribution and expression of Na,K-ATPase (NKA) in gill and kidney of the pufferfish were examined and the osmolality, [Na+] and [Cl-] of the blood were assayed. The lowest levels of both relative protein abundance and activity were found to be exhibited in the BW group, and higher levels in the SW group than FW group. In all salinities, branchial NKA immunoreactivity was found in epithelial cells of the interlamellar region of the filament and not on the lamellae. Relative abundance of kidney NKA alpha-subunit, as well as the NKA activity, was found to be higher in the FW pufferfish than fish in BW or SW. Renal NKA appeared in the epithelial cells of distal tubules, proximal tubules, and collecting tubules, but not in glomeruli, in fish groups of various salinities. Plasma osmolality and chloride levels were significantly lower in FW pufferfish than those in BW and SW, whereas plasma sodium did not differ among the groups. Although identical distributions of NKA were found in either gill or kidney of FW-, BW- or SW-acclimated spotted green pufferfish, differential NKA expression in fish of various salinity groups was associated with physiological homeostasis (stable blood osmolality), and illustrated the impressive osmoregulatory ability of this freshwater and estuarine species in response to salinity challenge. (C) 2004 Elsevier Inc. All rights reserved.