ISOLATION OF AN ESCHERICHIA-COLI MUTANT DEFICIENT IN THIOREDOXIN REDUCTASE
ISOLATION OF AN ESCHERICHIA-COLI MUTANT DEFICIENT IN THIOREDOXIN REDUCTASE
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DOI：
10.1128/jb.129.2.967-972.1977
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发表时间：
1977-01-01
影响因子：
3.2
通讯作者：
FUCHS, J
中科院分区：
文献类型：
--
作者：
FUCHS, J
分离到一株硫氧还蛋白还原酶缺陷的大肠杆菌突变株，并对其进行了部分鉴定。该突变体在体外没有检测到硫氧还蛋白还原酶活性，但在体内对核苷酸的还原没有缺陷。在通过乙醚处理而被渗透的细胞中，核糖核苷二磷酸的还原可以利用谷胱甘肽作为替代还原系统。
A mutant of E. coli defective in thioredoxin reductase was isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. In cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.