Electrochemical properties of an interface between titanium and fibroblasts L929
Electrochemical properties of an interface between titanium and fibroblasts L929
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DOI：
10.1016/s0013-4686(02)00684-9
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发表时间：
2002-12
影响因子：
6.6
通讯作者：
S. Hiromoto;K. Noda;T. Hanawa
中科院分区：
文献类型：
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作者：
S. Hiromoto;K. Noda;T. Hanawa
在培养和不培养鼠成纤维细胞L929的情况下对钛进行各种电化学测量，以表征细胞对钛和细胞之间的界面电化学性质的影响。在L929电池中，钛的开路电位降低，这是由于阴极电流密度的降低所指示的阴极和阳极反应之间的平衡电位的移动引起的。在阴极电位阶跃试验中，L929电池的电流密度下降滞后于峰值电流密度，表明分子和离子的扩散率随电池的增加而降低。此外，交流阻抗测量和数据近似的电气等效电路模型显示，电路元件的生物分子吸附层的扩散阻力增加与L929细胞。因此，细胞对界面性质的影响是由于生物分子密度的增加而延迟通过生物分子吸附层的扩散，细胞外基质由细胞产生的蛋白质和糖胺聚糖组成。
Various electrochemical measurements were performed on titanium with and without culturing murine fibroblasts L929 to characterize the effects of cells on interface electrochemical properties between titanium and cells. Open-circuit potential of titanium decreased with L929 cells, which was caused by the shift of equilibrium potential between cathodic and anodic reactions indicated by the decrease in cathodic current density with L929 cells. In cathodic potential step test, the decrease in current density following to the peak current density was delayed with L929 cells, indicating that diffusivity of molecules and ions decreased with the cells. In addition, alternating current impedance measurement and data approximation to the electrical equivalent circuit model revealed that the circuit element for diffusion resistance of biomolecule adsorption layer increased with L929 cells. Consequently, the effect of cells on the interface property is the retardation of diffusion through the biomolecule adsorption layer due to the increase in biomolecule density with extracellular matrix consisting with proteins and glycosaminoglycans generated by the cells.