Insertional mutagenesis of the maize P gene by intragenic transposition of Ac.
Insertional mutagenesis of the maize P gene by intragenic transposition of Ac.
复制标题
DOI：
--
复制
发表时间：
1992-05
期刊：
影响因子：
3.3
通讯作者：
P. Athma;Erich Grotewold;Thomas Peterson
中科院分区：
文献类型：
--
作者：
P. Athma;Erich Grotewold;Thomas Peterson
玉米P基因的P-rr等位基因调控果皮、颖壳和其他花器官中黄烷-4-醇类色素的合成。我们鉴定了21个P等位基因，这些P等位基因来自三个已知位置的Ac基因内转位。Ac转位可以发生在P基因的任何一个方向上，并且没有明显的最小距离：在一例中，Ac转位距离其原始插入位点仅6bp。然而，转座Ac元件的分布具有明显的非随机性：在来自单个Ac供体的19个转座Ac元件中，15个插入到P的5‘端的1.1kb区域，而没有插入到相邻的3.2kb内含子区域。所有ac插入都会影响果皮和果穗颖壳的色素沉着，进一步证明P-rr等位基因包含果皮和果穗颖片色素沉着所需的单个基因。插入的ac元件的分布和受每个等位基因制约的表型表明，P-rr的结构与先前确定的分子结构相似。讨论了换位交流元件非随机分布的可能原因。
The P-rr allele of the maize P gene regulates the synthesis of pigments derived from flavan-4-ol in the pericarp, cob glumes and other floral organs. We characterized 21 P alleles derived by intragenic transposition of Ac from three known positions. Ac transpositions can occur in either direction in the P gene, and with no apparent minimum distance: in one case Ac transposed just 6 bp from its original insertion site. However, the distribution of transposed Ac elements was markedly nonrandom: of 19 transposed Ac elements derived from a single Ac donor, 15 were inserted in a 1.1-kb region at the 5' end of P, while none had inserted in an adjacent 3.2-kb intronic region. All of the Ac insertions affect both pericarp and cob glume pigmentation, providing further evidence that the P-rr allele contains a single gene required for both pericarp and cob glume pigmentation. The distribution of the inserted Ac elements and the phenotype conditioned by each allele suggests a structure of P-rr which is similar to that previously determined molecularly. Possible explanations for the nonrandom distribution of transposed Ac elements are discussed.