Laser-induced autofluorescence study of caries model in vitro
Laser-induced autofluorescence study of caries model in vitro
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DOI：
10.1007/s10103-005-0365-7
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发表时间：
2006-04-01
影响因子：
2.1
通讯作者：
Avramov, L
中科院分区：
文献类型：
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作者：
Borisova, E;Uzunov, T;Avramov, L
用337 nm氮激光照射离体牙，在牙釉质脱矿和龋菌群植物群进入龋损的过程中，测量其激光诱导自体荧光光谱。从健康牙齿获得的光谱包括在480-500 nm处的强度最大值和在430-450 nm处的次最大值。在龋病形成过程中，我们观察到在430-450 nm处的强度增加，并且在红色光谱区域-在590-650 nm处出现两个最大值。在430-450 nm处的强度增加与牙齿脱矿有关。细菌的存在和它们的代谢产物诱导在350-420 nm的UV-蓝色光谱区域中的吸收的增加和在590-650 nm的长波光谱区域中的荧光信号的出现。从组织光学的观点来看，这些结果允许龋被认为是由两种不同的现象组成的-组织破坏和细菌植物群及其代谢产物增加。该结果可用于根据其荧光特性获得更完整的龋齿形成图像。
Laser-induced autofluorescence spectra of teeth irradiated by a 337 nm nitrogen laser were measured during in vitro caries formation through initial enamel demineralization and introducing of carious bacterial flora in the lesions developed. Spectra obtained from sound teeth consist of an intensive maximum at 480-500 nm and secondary maximum at 430-450 nm. In the process of caries formation, we observed an increase in the intensity at 430-450 nm and the appearance of two maxima in the red spectral region-at 590-650 nm. The intensity increase at 430-450 nm was related to the tooth demineralization. Bacteria presence and their metabolism products induced an increase in the absorption in the UV-blue spectral region at 350-420 nm and the appearance of a fluorescence signal in the long-wave spectral region at 590-650 nm. From the point of view of tissue optics, these results allow caries to be considered as consisting of two different phenomena-tissue destruction and bacterial flora and its metabolism products increase. The results could be used to obtain a more complete picture of caries formation on the base of its fluorescent properties.