Biological determinants of and reference values for plasma interleukin-8, monocyte chemoattractant protein-1, epidermal growth factor, and vascular endothelial growth factor: Results from the STANISLAS cohort
Biological determinants of and reference values for plasma interleukin-8, monocyte chemoattractant protein-1, epidermal growth factor, and vascular endothelial growth factor: Results from the STANISLAS cohort
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DOI：
10.1373/clinchem.2005.055798
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发表时间：
2006-03-01
影响因子：
9.3
通讯作者：
Visvikis-Siest, S
中科院分区：
文献类型：
--
作者：
Berrahmoune, H;Lamont, JV;Visvikis-Siest, S
背景：白细胞介素-8 （IL-8）、单核细胞化学吸引蛋白-1 （MCP-1）、表皮生长因子（EGF）和血管内皮生长因子（VEGF）被认为与炎症、血管重塑或生长失调相关的各种疾病有关。此外，这些细胞因子的血浆浓度升高似乎提供了有用的诊断和预后信息。因此，我们研究了哪些因素对血浆IL-8、MCP-1、EGF和VEGF浓度的生物学变化影响最大。方法：我们使用Evidence(R)生物芯片阵列分析仪定量分析STANISLAS家族研究中304名儿童（年龄范围4-17岁）和540名成人（年龄范围18-55岁）的血浆IL-8、MCP-1、EGF和VEGF浓度。我们还计算了4种细胞因子的参考区间。结果：我们发现以下与血浆标志物浓度的关系：年龄、中性粒细胞计数和葡萄糖浓度与IL-8浓度在儿童和成人中呈正相关，吸烟和血小板计数在成人中也是如此。MCP-1浓度与儿童和成人的年龄和吸烟有关，与儿童的单核细胞计数有关，与成人的性别和红细胞压积有关。EGF浓度与儿童血小板计数和成人单核细胞计数和葡萄糖有关。VEGF浓度与儿童和成人的年龄以及成人的血小板计数和丙氨酸转氨酶活性有关。结论：我们IL-8、MCP-1、EGF和VEGF的结果可能有助于解释患者的实验室结果，并有助于理解这些细胞因子在生理条件下的浓度调节。(C) 2006美国临床化学学会。
Background: Interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF) are known to be involved in various diseases related to inflammation, vascular remodeling, or growth deregulation. In addition, increases in plasma concentrations of these cytokines appear to provide useful diagnostic and prognostic information. We therefore investigated which factors most strongly influence the biological variations of plasma IL-8, MCP-1, EGF, and VEGF concentrations.Methods: We used the Evidence(R) biochip array analyzer to quantify plasma IL-8, MCP-1, EGF, and VEGF concentrations in a subsample of 304 children (age range, 4-17 years) and 540 adults (age range, 18-55 years) from the STANISLAS family study. We also calculated reference intervals for the 4 cytokines.Results: We found the following associations with plasma marker concentrations: Age, neutrophil count, and glucose concentration were positively associated with IL-8 concentrations in children and adults, as were smoking and platelet count in adults. MCP-1 concentrations were associated with age and smoking in both children and adults, monocyte count in children, and sex and hematocrit in adults. EGF concentrations were associated with platelet count in children and monocyte count and glucose in adults. VEGF concentrations were associated with age in children and adults and platelet count and alanine aminotransferase activity in adults.Conclusion: Our results for IL-8, MCP-1, EGF, and VEGF may be useful for interpretation of patients' laboratory results and for understanding the regulation of concentrations of these cytokines in physiologic conditions. (C) 2006 American Association for Clinical Chemistry.