Antibody reactivity against thyroid peroxidase and myeloperoxidase in autoimmune thyroiditis and systemic vasculitis
Antibody reactivity against thyroid peroxidase and myeloperoxidase in autoimmune thyroiditis and systemic vasculitis
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DOI：
10.1046/j.1365-3083.1997.d01-90.x
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发表时间：
1997-07-01
影响因子：
3.7
通讯作者：
Soppi, E
中科院分区：
文献类型：
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作者：
Haapala, AM;Hyoty, H;Soppi, E
通过分析199例TPO抗体阳性和145例MPO抗体阳性血清与TPO和MPO分子的结合，评价了甲状腺过氧化物酶（TPO）和髓过氧化物酶（MPO）分子之间的潜在交叉反应性。然后选择具有不同TPO-MPO抗体结果的6名自身免疫性甲状腺炎（AITD）患者和4名系统性血管炎（SV）患者的血清进行进一步分析。6例AITD患者酶免疫测定（EIA）均检测出TPO抗体，4例同时检测出MPO抗体。在AITD患者中，将天然MPO加入血清稀释液中不能抑制抗体与TPO的结合，这表明可能的交叉反应表位暴露在变性的MPO分子中。同样，系统性血管炎患者的MPO ab反应性不能被天然TPO抑制。为了研究TPO和MPO抗体是否识别线性表位，分析了抗体与合成的TPO和MPO肽的结合。几种TPO和MPO肽是反应性的，包括与TPO和MPO抗体阳性血清反应的肽。最具交叉反应性的肽之一在TPO中含有AA 586-601，还显示出与MPO（AA 594-609）特别高的AA同源性（88%）。结果表明，TPO和MPO分子含有交叉反应性表位，这些表位暴露在变性分子中，因此可能导致固相EIA测定中的假阳性抗体结果。
Potential cross-reactivity between thyroid peroxidase (TPO) and myeloperoxidase (MPO) molecules was evaluated by analysing the binding of 199 TPO antibody- and 145 MPO antibody- positive sera to TPO and MPO molecules. Sera from six patients with autoimmune thyroiditis (AITD) and four patients with systemic vasculitis (SV) with different TPO-MPO antibody findings were then chosen for further analyses. All six patients with AITD had TPO antibodies in enzyme immunoassay (EIA) and lour of them had simultaneously MPO antibodies in EIA. In AITD patients antibody binding to TPO could not be inhibited by adding native MPO to the serum diluent, suggesting that the possible cross-reactive epitopes were exposed in the denaturated MPO molecule. Similarly, the MPO ab reactivity of patients with systemic vasculitis could not be inhibited by native TPO. To study whether TPO and MPO antibodies recognize linear epitopes, the binding of antibodies to synthetic TPO and MPO peptides was analysed, Several TPO and MPO peptides were reactive, including peptides reacting with both TPO and MPO antibody-positive sera. One of the most crossreactive peptides contained AA 586-601 in TPO, showing also particularly high AA homology (88%) with MPO (AA 594-609). The results suggest that TPO and MPO molecules contain cross-reactive epitopes that are exposed in denaturated molecules and may thus cause false positive antibody findings in solid phase EIA assays.