MATK DNA-SEQUENCES AND PHYLOGENETIC RECONSTRUCTION IN SAXIFRAGACEAE S-STR
MATK DNA-SEQUENCES AND PHYLOGENETIC RECONSTRUCTION IN SAXIFRAGACEAE S-STR
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DOI：
10.2307/2419718
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发表时间：
1994-01-01
影响因子：
1
通讯作者：
SOLTIS, DE
中科院分区：
文献类型：
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作者：
JOHNSON, LA;SOLTIS, DE
matK是一个成熟酶编码基因，位于叶绿体基因trnK的内含子内，比较DNA测序的系统发育效用进行了评估，使用虎耳草科属。对该家族的两个成员Sullivantia sullivantii和Saxifraga integrifolia的整个matK基因进行测序。这两个物种之间的碱基替换率的比较表明，matK的进化速度比rbcL快约3倍。matK内的754个碱基对的比较测序，随后进行了25个属的虎耳草科。str.和两个外群类群。总结了这754个碱基对区域测序的31个分类群，40%的碱基位置是可变的，15.6%是潜在的信息。还检测到三个或六个碱基对的五个插入/缺失事件。偏度和随机化测试都表明matK数据集中存在显著的非随机结构。简约分析提供了72个最简约的树的223个步骤（不包括autapomorphies）的一致性指数为0.565。几个良好的支持集团的属是高度一致的关系，建议由其他两个叶绿体DNA数据集：叶绿体DNA限制性位点和rbcL序列。
Comparative DNA sequencing of matK, a maturase coding gene located within the intron of the chloroplast gene trnK, was evaluated for phylogenetic utility using genera of Saxifragaceae s. str. The entire matK gene was sequenced for two members of the family, Sullivantia sullivantii and Saxifraga integrifolia. Comparison of base substitution rates between these two species indicated that matK evolves approximately three-fold faster than rbcL. Comparative sequencing of 754 base pairs within matK was subsequently conducted using 25 genera in Saxifragaceae s. str. arid two outgroup taxa. Summed over the 31 taxa sequenced for this 754 base pair region, 40% of the base positions were variable and 15.6% were potentially informative. Five insertion/deletion events of three or six base pairs were also detected. Skewness and randomization tests both suggest that significant non-random structure is present in the matK data set. Parsimony analyses provided 72 most parsimonious trees of 223 steps (excluding autapomorphies) with a consistency index of 0.565. Several well-supported groups of genera are highly concordant with relationships suggested by two other chloroplast DNA data sets: chloroplast DNA restriction sites and rbcL sequences.