Association study between miR-149 gene polymorphism and nasopharyngeal carcinoma
Association study between miR-149 gene polymorphism and nasopharyngeal carcinoma
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miR-149基因多态性与鼻咽癌的关联研究
DOI：
10.3892/br.2013.97
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发表时间：
2013-07-01
影响因子：
2.3
通讯作者：
He, Zhiwei
中科院分区：
文献类型：
--
作者：
Huang, Guo-Liang;Lu, Yan;He, Zhiwei
miR-149基因上的单核苷酸多态性（SNP）rs 2292832与癌症风险之间的关联研究先前已在几种类型的癌症中进行了分析。本研究旨在探讨miR-149基因多态性与鼻咽癌发病风险的关系。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对158例鼻咽癌患者和242例健康人的miR-149基因多态性进行检测。与癌症风险和临床病理特征的关联通过0.2检验进行分析。NPC患者和健康对照者的miR-149基因多态性在基因型（CC vs. CT vs. TT，P=0.427; CT vs. TT，P=0.247; CC vs. TT，P=0.323）或等位基因分析（P=0.216）中均无显著差异。除临床分期外，基因型与临床病理参数之间无显著性差异。在显性模型（CC vs. CT vs. TT，P=0.026）和共显性模型（CC vs. TT，P=0.030）下，观察到临床I-II期的CC分布显著高于III-IV期。本研究结果提示，miR-149基因CC型参与了鼻咽癌的进展和发展，而不是鼻咽癌的发生。
Association studies between single-nucleotide polymorphism (SNP) rs2292832 on miR-149 gene and cancer risk have been previously analyzed in several types of cancer. The aim of this study was to evaluate the association between miR-149 polymorphism and risk of nasopharyngeal carcinoma (NPC). miR-149 gene polymorphism was genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) in 158 patients with NPC and 242 healthy individuals. Associations with cancer risk and clinicopathological characteristics were analyzed by.2 test. No significant difference was observed for miR-149 gene polymorphism in NPC patients and healthy controls in either genotype (P=0.427 for CC vs. CT vs. TT, P=0.247 for CT vs. TT and P=0.323 for CC vs. TT, respectively) or allelic analysis (P=0.216). No significant difference was noted between the genotypes and the clinicopathological parameters examined with the exception of clinical stage. A significantly higher CC distribution in clinical stage I-II compared with III-IV was observed under the dominant model (CC vs. CT vs. TT, P=0.026) and the co-dominant model (CC vs. TT, P=0.030). The results of this study suggested that the CC genotype of miR-149 contributes to the progression and development, rather than the initiation of NPC.