IDENTIFICATION OF A MAJOR KERATINOCYTE CELL-ENVELOPE PROTEIN, LORICRIN
IDENTIFICATION OF A MAJOR KERATINOCYTE CELL-ENVELOPE PROTEIN, LORICRIN
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DOI：
10.1016/0092-8674(90)90073-n
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发表时间：
1990-06-15
期刊：
影响因子：
64.5
通讯作者：
ROOP, DR
中科院分区：
文献类型：
--
作者：
MEHREL, T;HOHL, D;ROOP, DR
在表皮细胞角化过程中，一层共价交联蛋白沉积在质膜的细胞质表面，形成细胞膜。我们分离并鉴定了编码小鼠表皮细胞主要分化产物的cDNA克隆，其氨基酸组成与纯化的细胞膜相似。该基因的转录本仅限于颗粒层，与分化特异性角蛋白K1和K10一样丰富。针对C端肽的抗血清将该蛋白定位于颗粒层中的离散颗粒中，随后定位于角层细胞的外围。免疫荧光和免疫电子显微镜仅在纯化细胞包膜的内表面检测到该表位。综上所述，这些结果表明，它是细胞膜的主要成分。根据其推测的功能，这种蛋白质被命名为氯氯蛋白。
During epidermal cell cornification, the deposition of a layer of covalently cross-linked protein on the cytoplasmic face of the plasma membrane forms the cell envelope. We have isolated and characterized cDNA clones encoding a major differentiation product of mouse epidermal cells, which has an amino acid composition similar to that of purified cell envelopes. Transcripts of this gene are restricted to the granular layer and are as abundant as the differentiation-specific keratins, K1 and K10. An antiserum against a C-terminal peptide localizes this protein in discrete granules in the stratum granulosum and subsequently at the periphery of stratum cornerum cells. Immunofluorescence and immunoelectron microscopy detect this epitope only on the inner surface of purified cell envelopes. Taken together, these results suggest that it is a major component of cell envelopes. On the basis of its presumed function, this protein is named loricrin.