PCR detection of human papillomavirus: Comparison between MY09/MY11 and GP5+/GP6+ primer systems
PCR detection of human papillomavirus: Comparison between MY09/MY11 and GP5+/GP6+ primer systems
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DOI：
10.1128/jcm.35.6.1304-1310.1997
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发表时间：
1997-06-01
影响因子：
9.4
通讯作者：
Burk, RD
中科院分区：
文献类型：
--
作者：
Qu, WM;Jiang, G;Burk, RD
人乳头瘤病毒 (HPV) 是宫颈癌的病原体，也是女性中最常见的性传播疾病，HPV 基因组的 PCR 扩增是检测宫颈阴道 HPV 的最敏感方法。我们比较了两种最常用的 PCR 引物组 My09/MY11 (MY-PCR) 和 GP5+/GP6+ (GPC-PCR)，用于检测 208 例宫颈阴道灌洗样本中的 HPV DNA女性，使用了针对 39 种不同 HPV 类型的寡核苷酸探针，两种引物组均扩增了广泛的 HPV 基因型，并检测到相似的总体患病率，分别为 45%（208 例中的 94 例）和 43%（208 例中的 89 例）。MY-PCR 系统检测到 30 个样本中的 27 个（90%）具有多种 HPV 类型，而 GP+-PCR 系统检测到 30 个样本中的 14 个（47%）具有多种 HPV 类型。两种引物系统对 HPV 35、53 和 61 型的检测存在差异，质粒模板的连续稀释表明 MY-PCR 检测的 HPV 35 型扩增量下降了 3 个对数，GP+-PCR 检测的 HPV 53 型和 61 型检测结果下降了 3 个对数。这些结果表明，尽管MY-PCR和GP+-PCR在一组临床样本中确定了几乎相同的HPV患病率，但在特定类型和多种类型感染的检测中发现了差异，在比较研究之间的数据和/或设计新的研究或临床试验时，应考虑PCR系统检测临床样本中HPV的敏感性和特征的差异。
Human papillomavirus (HPV) is an etiologic agent of cervical cancer and is the most common sexually transmitted disease in women, PCR amplification of HPV genomes is the most sensitive method for the detection of cervicovaginal HPV, We have compared the two most commonly used PCR primer sets, My09/MY11 (MY-PCR) and GP5+/GP6+ (GPC-PCR), for the detection of HPV DNA in cervicovaginal lavage samples from 208 women, Oligonucleotide probes for 39 different HPV types were used, Both primer sets amplified a wide spectrum of HPV genotypes and detected similar overall prevalences of 45% (94 of 208) and 43% (89 of 208), respectively, The MY-PCR system detected 27 of 30 (90%) samples with multiple HPV types, whereas the GP+-PCR system detected 14 of 30 (47%) samples with multiple HPV types, Differences in the detection of HPV types 35, 53, and 61 were noted between the two primer systems, Serial dilution of plasmid templates indicated a 3-log decrease in the amplification of HPV type 35 by MY-PCR and HPV types 53 and 61 by GP+-PCR. These results indicate that although the MY-PCR and GP+-PCR identified nearly equivalent prevalences of HPV in a set of clinical samples, differences in the detection of specific types and infections with multiple types were found, Differences in the sensitivities and characteristics of the PCR systems for the detection of HPV within clinical samples should be considered when comparing data between studies and/or in designing new studies or clinical trials.