Engineering a biospecific communication pathway between cells and electrodes
Engineering a biospecific communication pathway between cells and electrodes
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DOI：
10.1073/pnas.0504349103
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发表时间：
2006-02-14
影响因子：
11.1
通讯作者：
Mrksich, M
中科院分区：
文献类型：
--
作者：
Collier, JH;Mrksich, M
将哺乳动物细胞的细胞活动转化为可测量的电子信号的方法在许多生物技术应用中非常重要，包括生物传感器、细胞阵列和其他基于细胞的设备。这份手稿描述了一种在底层衬底中功能性地整合细胞活动和电子过程的方法。这些细胞被改造成带有细胞表面嵌合受体的细胞，该受体呈现非哺乳动物酶角质酶。这种细胞表面角质酶在酶底物自组装单分子层上的作用将非电活性的羟基苯酯转换为电活性的对苯二酚，提供了可以用循环伏安法识别的电活性。通过这种方式，细胞表面的酶活性被转化为电子信号。开发直接将细胞的活动与材料连接起来的策略，对于实现将生物和非生物成分结合在一起的广泛类别的混合微系统将是重要的。
Methods for transducing the cellular activities of mammalian cells into measurable electronic signals are important in many biotechnical applications, including biosensors, cell arrays, and other cell-based devices. This manuscript describes an approach for functionally integrating cellular activities and electrical processes in an underlying substrate. The cells are engineered with a cell-surface chimeric receptor that presents the nonmammalian enzyme cutinase. Action of this cell-surface cutinase on enzyme substrate self-assembled monolayers switches a nonelectroactive hydroxyphenyl ester to an electroactive hydroquinone, providing an electrical activity that can be identified with cyclic voltammetry. In this way, cell-surface enzymatic activity is transduced into electronic signals. The development of strategies to directly interface the activities of cells with materials will be important to enabling a broad class of hybrid microsystems that combine living and nonliving components.