Kinetics of prion protein accumulation in the CNS of mice with experimental scrapie.
Kinetics of prion protein accumulation in the CNS of mice with experimental scrapie.
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患有实验性痒病的小鼠中枢神经系统中朊病毒蛋白积累的动力学。
DOI：
10.1097/00005072-199912000-00005
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发表时间：
1999
影响因子：
3.2
通讯作者：
S. DeArmond
中科院分区：
文献类型：
--
作者：
Jörg Tatzelt;D. Groth;M. Torchia;S. B. Prusiner;S. DeArmond
研究了CD-1小鼠脾和脑内PrP（Sc）和不溶性PrP积累的动力学。小鼠在脑内接种RML朊病毒，并在接种和发病之间的不同时间（约130天）实施安乐死。在接种后第49天和第70天分别用组织印迹法和免疫印迹法在脑组织中首次检测到蛋白酶抗性PrP(Sc), PrP 27-30。脾中PrP 27-30在接种后28天首次用免疫印迹法检测。与PrP 27-30一样，在接种后第70天和第28天，在大脑和脾脏中首次检测到洗涤剂不溶性PrP的大量增加。此外，接种后70天开始检测到洗涤剂可溶性PrP的逐渐增加。进一步表征洗涤剂溶性和不溶性PrP与蛋白酶敏感PrP（Sc）和朊病毒的感染性将是相当有趣的。
The kinetics of PrP(Sc) and insoluble PrP accumulation in the spleens and brains of CD-1 mice were studied. The mice were inoculated intracerebrally with RML prions and euthanized at various times between inoculation and the onset of illness at approximately 130 days. Protease-resistant PrP(Sc), PrP 27-30, was first detected in brain by histoblotting 49 days after inoculation and by Western immunoblotting at 70 days. In spleen, PrP 27-30 was first detected by Western immunoblotting at 28 days after inoculation. Like PrP 27-30, substantial increases in detergent-insoluble PrP were first detected at 70 days after inoculation in brain and 28 days in spleen. In addition, a progressive increase in detergent-soluble PrP was detected beginning 70 days after inoculation. Further characterization of detergent soluble and insoluble PrP with respect to protease-sensitive PrP(Sc) and prion infectivity will be of considerable interest.