Unexpected problem in aphid DNA barcoding by universal primers
Unexpected problem in aphid DNA barcoding by universal primers
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DOI：
10.1111/j.1479-8298.2011.00473.x
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发表时间：
2012-01
影响因子：
0.9
通讯作者：
Wonhoon Lee;Seunghwan Lee
中科院分区：
文献类型：
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作者：
Wonhoon Lee;Seunghwan Lee
DNA条形码（线粒体COI）序列允许蚜虫的物种鉴定。在本研究中，我们在麦长管蚜的部分DNA序列中发现了DNA条形码问题。五S结果表明，麦长管蚜的5个基因序列与其它种的基因序列差异平均为32.60%，经BLAST检索，这5个基因序列与蚜虫属Aphidius、麻黄素属Ephedrus和草地螟属Praon spp.（膜翅目：茧蜂科）。基于这些结果，我们得出结论，蚜虫DNA条形码中使用的通用引物可以从宿主蚜虫内的寄生虫物种中扩增条形码序列。
DNA barcode (mitochondrial COI) sequences have allowed for species identification of aphids. In this study, we newly found a DNA barcoding problem in a part of the DNA sequences for Sitobion avenae. Five S. avenae individuals showed differences of, on average, 32.60% in the DNA sequences from other conspecific individuals, and a BLAST search revealed that the five sequences are similar to those of aphid parasitoids such as Aphidius, Ephedrus and Praon spp. (Hymenoptera: Braconidae). Based on these results, we concluded that the universal primers used in aphid DNA barcodes can amplify barcode sequences from parasitoid species within host aphids.