Controlled magnetic nanofiber hydrogels by clustering ferritin.
Controlled magnetic nanofiber hydrogels by clustering ferritin.
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DOI：
10.1021/la802155a
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发表时间：
2008-11
期刊：
影响因子：
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通讯作者：
M. Shin;S. I. Kim;Seon Jeong Kim;Sang Yoon Park;Y. H. Hyun;Youngpak Lee;K. Lee;S. Han;D. Jang
中科院分区：
文献类型：
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作者：
M. Shin;S. I. Kim;Seon Jeong Kim;Sang Yoon Park;Y. H. Hyun;Youngpak Lee;K. Lee;S. Han;D. Jang
利用静电纺丝技术，根据溶液的混合温度，制备了具有不同大小铁蛋白簇的生物相容性水凝胶。以聚乙烯醇（PVA）为聚合物基体制备纳米复合材料。通过热手段，我们控制了主体PVA水凝胶和铁蛋白生物纳米颗粒上的蛋白质壳之间的相互作用，以改变铁蛋白簇的大小和浓度。铁蛋白的聚类是基于铁蛋白的蛋白质壳的部分解折叠。通过研究PVA/铁蛋白纳米纤维的磁性能，根据PVA/铁蛋白溶液的混合温度，我们证实了铁蛋白的聚集过程与PVA/铁蛋白纳米纤维的超顺磁性和磁共振成像（MRI）对比度的变化有关。具有不同空间分布的铁蛋白纳米颗粒的PVA/铁蛋白纳米水凝胶可用作基于MRI的非侵入性可检测的细胞培养支架和人工肌肉，因为它们具有改进的超顺磁性。
We have fabricated biocompatible nanofiber hydrogels with diverse sizes of ferritin clusters according to the mixing temperature of solutions employing electrospinning. Poly(vinyl alcohol) (PVA) was used as a polymeric matrix for fabricating nanocomposites. By thermal means we controlled the interaction between the host PVA hydrogel and the protein shell on ferritin bionanoparticles to vary the size and concentration of ferritin clusters. The clustering of ferritin was based on the partial unfolding of a protein shell of ferritin. By studying the magnetic properties of the PVA/ferritin nanofibers according to the mixing temperature of the PVA/ferritin solutions, we confirmed that the clustering process of the ferritin was related to changes in the superparamagnetic properties and magnetic resonance imaging (MRI) contrast of the PVA/ferritin nanofibers. PVA/ferritin nanofiber hydrogels with diverse spatial distributions of ferritin nanoparticles are applicable as MRI-based noninvasive detectable cell culture scaffolds and as artificial muscles because of their improved superparamagnetic properties.