INDUCTION OF CYTOSOLIC PHOSPHOLIPASE A(2) IN HUMAN-LEUKOCYTES BY LIPOPOLYSACCHARIDE
INDUCTION OF CYTOSOLIC PHOSPHOLIPASE A(2) IN HUMAN-LEUKOCYTES BY LIPOPOLYSACCHARIDE
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DOI：
10.1111/j.1432-1033.1994.tb19048.x
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发表时间：
1994-08-01
期刊：
影响因子：
--
通讯作者：
SCHEUER, W
中科院分区：
文献类型：
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作者：
RODEWALD, E;TIBES, U;SCHEUER, W
脂多糖刺激外周血白细胞可导致炎性细胞因子的合成，包括IL-1β、IL-6、肿瘤坏死因子-α和前列腺素E(2)，与磷脂酶A(2)的活性增加有关。哺乳动物细胞含有多种磷脂酶A(2)亚型，包括14 kDa的分泌型磷脂酶A(2)亚型和最近报道的高分子量胞浆亚型，一般认为在炎症反应过程中，分泌型磷脂酶A(2)被激活。然而，我们没有检测到分泌型磷脂酶A(2)及其相应的mRNA在内毒素诱导的激活后，我们发现外周血白细胞激活后胞浆磷脂酶A(2)的mRNA水平比未刺激的对照组增加。我们的结果表明，胞浆磷脂酶A(2)而不是分泌型磷脂酶A(2)可能是内毒素诱导的人外周血白细胞炎性级联反应的中介。
Stimulation of peripheral blood leukocytes with lipopolysaccharide results in the synthesis of inflammatory cytokines including interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha and prostaglandin E(2) correlating with an increase in phospholipase A(2) activity. Mammalian cells contain several phospholipase A(2) isoforms including the 14-kDa secretory isoform and the more recently described high-molecular-mass cytosolic isoform, It is commonly believed that during inflammatory responses secretory phospholipase A(2) becomes activated. However, we could not detect secretory phospholipase A(2) nor its corresponding mRNA after lipopolysaccharide-induced activation By contrast, we found increased mRNA levels for cytosolic phospholipase A(2) following activation of peripheral blood leukocytes when levels were compared to non-stimulated controls. Our results demonstrate that cytosolic phospholipase A(2), rather than the secretory isoform may be the mediator of the lipopolysaccharide-induced inflammatory cascade in human peripheral blood leukocytes.