A Handheld High-Sensitivity Micro-NMR CMOS Platform With B-Field Stabilization for Multi-Type Biological/Chemical Assays
A Handheld High-Sensitivity Micro-NMR CMOS Platform With B-Field Stabilization for Multi-Type Biological/Chemical Assays
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DOI：
10.1109/jssc.2016.2591551
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发表时间：
2017-01-01
影响因子：
5.4
通讯作者：
Martins, Rui P.
中科院分区：
文献类型：
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作者：
Lei, Ka-Meng;Heidari, Hadi;Martins, Rui P.
我们报告了一种与多种类型的生物/化学芯片实验室分析兼容的微型核磁共振（NMR）系统。该系统统一在手持式天平（尺寸：14 x 6 x 11 cm（3），重量：1.4 kg）中，能够从2.5 μ L样品中检测< 100 pM的粪肠球菌衍生DNA。该系统的关键部件是用于核磁化的便携式磁体（0.46 T，1.25 kg）、用于I/O接口的系统PCB、用于系统控制的FPGA、用于调整磁场（B）的电流驱动器和用0.18 μ m CMOS制造的硅芯片。后者集成了电流模式垂直霍尔传感器和低噪声读出电路，有助于闭环B场稳定（2 mT -> 0.15 mT），否则B场会随着温度或样本位移而波动。该系统与带有平面线圈的动态B场收发器一起用于微核磁共振分析和热控制，该系统演示了：1）选择性生物目标精确定位; 2）蛋白质状态分析; 3）溶剂聚合物动力学，分别适用于医疗保健，食品和胶体应用。与商业NMR分析产品（Bruker mq-20）相比，该平台大大减少了样品消耗（120倍）、硬件体积（175倍）和重量（96倍）。
We report a micro-nuclear magnetic resonance (NMR) system compatible with multi-type biological/chemical lab-on-a- chip assays. Unified in a handheld scale (dimension: 14 x 6 x 11 cm(3), weight: 1.4 kg), the system is capable to detect < 100 pM of Enterococcus faecalis derived DNA from a 2.5 mu L sample. The key components are a portable magnet (0.46 T, 1.25 kg) for nucleus magnetization, a system PCB for I/O interface, an FPGA for system control, a current driver for trimming the magnetic (B) field, and a silicon chip fabricated in 0.18 mu m CMOS. The latter, integrated with a current-mode vertical Hall sensor and a low-noise readout circuit, facilitates closed-loop B-field stabilization (2 mT -> 0.15 mT), which otherwise fluctuates with temperature or sample displacement. Together with a dynamic-B-field transceiver with a planar coil for micro-NMR assay and thermal control, the system demonstrates: 1) selective biological target pinpointing; 2) protein state analysis; and 3) solvent-polymer dynamics, suitable for healthcare, food and colloidal applications, respectively. Compared to a commercial NMR-assay product (Bruker mq-20), this platform greatly reduces the sample consumption (120x), hardware volume (175x), and weight (96x).