Multiple resistance mechanisms to penoxsulam in Echinochloa crus-galli from China
Multiple resistance mechanisms to penoxsulam in Echinochloa crus-galli from China
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DOI：
10.1016/j.pestbp.2022.105211
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发表时间：
2022-08-27
影响因子：
4.7
通讯作者：
Ma, Hongju
中科院分区：
文献类型：
--
作者：
Feng, Tangqi;Peng, Qian;Ma, Hongju
培诺舒拉姆是防治紫锥藻（Echinochloa cross -galli， L.）的重要除草剂。测定。2个耐药群体17GA （R1）和16NXB （R2）对培诺舒拉姆的抗性分别为17倍和3倍。已知ALS基因中Trp-574-Leu的耐药突变和可能涉及GST的培诺舒兰代谢率的提高导致了R1群体对培诺舒兰的耐药。该种群对als抑制剂吡苯并辛、双嘧菌酸钠和生长素除草剂喹诺乐耐药，但对accase抑制剂quizalofp -对乙基和cyhalofp -丁基敏感。在R2群体中没有发现已知的ALS基因突变，使其对ALS抑制除草剂产生抗性。然而，在R2植株中，培诺舒兰的代谢是易感群体14YC （S0）植株的4倍左右。酶抑制剂胡椒酰丁醇、马拉硫磷和4-氯-7-硝基苯并恶二唑逆转了该人群对哌诺舒南的耐药性。GST和P450酶活性以及GST1-1、GST1-2、GST1-3、CYP81A18、CYP81A12、CYP81A21基因在R2群体中显著升高。这些结果表明，中国中部地区的十字花菜种群存在多种抗性机制，需要通过多种化学和非化学方法进行有效的抗性管理。
Penoxsulam is an important herbicide for the control of Echinochloa crus-galli (L.) P. Beauv. Two resistant populations 17GA (R1) and 16NXB (R2) showed 17- and 3-fold resistance to penoxsulam, respectively. A known resistance mutation of Trp-574-Leu in ALS gene and enhanced rates of penoxsulam metabolism likely involving GST contribute to penoxsulam resistance in R1 population. This population had resistance to the ALS-inhibitors pyribenzoxim and bispyribac-sodium and the auxin herbicide quinclorac, but was susceptible to ACCaseinhibitors quizalofop-p-ethyl and cyhalofop-butyl. No known mutations in the ALS gene conferring target site resistance to ALS-inhibiting herbicides were presented in R2 population. However, penoxsulam metabolism in R2 plants was about 4-fold greater than in susceptible population 14YC (S0) plants. The enzyme inhibitors piperonyl butoxide, malathion and 4-chloro-7-nitrobenzoxadiazole reversed penoxsulam resistance in this population. GST and P450 enzyme activities and the genes of GST1-1, GST1-2, GST1-3, CYP81A18, CYP81A12, CYP81A21 were increased significantly in R2 population. These results indicate that multiple resistance mechanisms had occurred in E. crus-galli populations in central China and resistance needs to be managed effectively by diverse chemical and non-chemical methods.