Lactobacillus plantarum Prevents the Upregulation of Adhesion Molecule Expression in an Experimental Colitis Model
Lactobacillus plantarum Prevents the Upregulation of Adhesion Molecule Expression in an Experimental Colitis Model
复制标题
植物乳杆菌防止实验性结肠炎模型中粘附分子表达的上调
DOI：
10.1007/s10620-009-1063-2
复制
发表时间：
2010-09-01
影响因子：
3.1
通讯作者：
Qin, Huan-Long
中科院分区：
文献类型：
--
作者：
Chu, Zhao-Xin;Chen, Hong-Qi;Qin, Huan-Long
背景：乳酸菌的摄入已被证明可以减轻实验性结肠炎的严重程度。乳酸菌对结肠炎的影响是否与调节白细胞进入炎症肠道有关尚不清楚。目的：探讨植物乳杆菌每日灌胃对结肠炎实验模型白细胞介素10 （IL-10）敲除小鼠淋巴细胞归巢和肠道炎症的影响。方法：两组10只IL-10基因敲除小鼠分别饲喂含植物乳杆菌1258的磷酸盐缓冲盐水或未修饰的载体4周。以两组10只野生型小鼠为对照。杀死后，用透射电子显微镜对肠道进行组织学评分和评估。免疫组化法检测粘膜寻址蛋白细胞粘附分子1 （MAdCAM-1）和细胞间粘附分子1 （ICAM-1）的表达。ELISA法检测促炎细胞因子、肿瘤坏死因子α (tnf - α)、干扰素γ (ifn - γ)水平。采用逆转录聚合酶链反应和Western blot检测CD3、alpha4beta7、ICAM-1和MAdCAM-1的表达水平。与未治疗的KO小鼠相比，植物治疗改善了KO小鼠的组织学损伤评分。与未治疗的KO小鼠相比，植物乳杆菌显著降低了治疗后的KO小鼠MAdCAM-1、ICAM-1、CD3和alpha4beta7的表达，但未影响tnf - α和ifn - γ的水平。与野生型小鼠相比，植物可干扰IL-10敲除小鼠粘附分子的上调，从而减轻结肠炎症状。
Background:Lactobacillus consumption has been shown to attenuate the severity of experimental colitis. Whether the effects of Lactobacillus on colitis are related to modulation of leukocyte recruitment into the inflamed intestine is unclear.Aims:To investigate the effect of Lactobacillus plantarum daily intragastric administration on lymphocyte homing and intestinal inflammation in interleukin 10 (IL-10) knockout mice, an experimental model of colitis.Methods:Two groups of ten IL-10 knockout mice were fed phosphate buffered saline containing Lactobacillus plantarum 1258 or unmodified vehicle for 4 weeks. Two groups of ten wild-type mice were used as controls. At killing, the bowels were histologically scored and evaluated by transmission electron microscopy. Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1) expression were determined by immunohistochemistry. The levels of proinflammatory cytokines, tumor necrosis factor alpha (TNF-alpha), and interferon gamma (IFN-gamma) were determined by ELISA. In addition, levels of CD3, alpha4beta7, ICAM-1, and MAdCAM-1 were determined by reverse-transcription polymerase chain reaction and Western blot.Results:L. plantarum treatment improved the histological damage score in KO mice compared to untreated KO mice. L. plantarum significantly attenuated the expression of MAdCAM-1, ICAM-1, CD3, and alpha4beta7, but did not affect the levels of TNF-alpha and IFN-gamma when treated KO mice were compared to untreated KO mice.Conclusions:L. plantarum interfered with the upregulation of adhesion molecules observed in IL-10 knockout mice compared to wild-type mice, attenuating the symptoms of colitis.