Complete chloroplast genome sequences of Rehmannia chingii, an endemic and endangered herb
Complete chloroplast genome sequences of Rehmannia chingii, an endemic and endangered herb
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特有濒危草本地黄叶绿体基因组全序列
DOI：
10.1007/s12686-016-0577-0
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发表时间：
2016-12-01
影响因子：
1.1
通讯作者：
Liu, Zhan-Lin
中科院分区：
文献类型：
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作者：
Zeng, Shuyun;Zhao, Jianhua;Liu, Zhan-Lin
本研究采用Illumina对端测序技术测定了稀有草本地黄（Rehmannia chingii）的全叶绿体基因组（cpDNA）序列。cpDNA长154,055 bp，包括一对倒置重复（IR）区（25,707 bp），一个大单拷贝（LSC）区（84,966 bp）和一个小单拷贝（SSC）区（17,675 bp）。叶绿体基因组包含133个独特基因，其中蛋白质编码基因88个，转移RNA基因37个，核糖体RNA基因8个，其中17个基因在IRs内复制，包括6个蛋白质编码基因，7个tRNA基因，4个rRNA基因。全基因组总A+T含量为62.0%，而LSC、SSC和IR区对应的A+T含量分别为64.0%、67.7%和56.8%。基于叶绿体基因组的系统发育分析表明，chingii与Lindenbergia philippensis亲缘关系较近。
The complete chloroplast genome (cpDNA) sequence of a rare herbaceous species, Rehmannia chingii, was determined by Illumina pair-end sequencing in this study. The cpDNA is 154,055 bp long, and includes a pair of inverted repeat (IR) regions (25,707 bp), one large single copy (LSC) region (84,966 bp), and one small single copy (SSC) region (17,675 bp). The chloroplast genome contains 133 unique genes, including 88 protein-coding genes, 37 transfer RNA genes, and 8 ribosomal RNA genes, 17 of which are duplicated within the IRs, including 6 protein-coding genes, 7 tRNA genes, and 4 rRNA genes. The overall A+T content of the whole genome is 62.0 %, while the corresponding values of the LSC, SSC and IR regions are 64.0, 67.7 and 56.8 %, respectively. A phylogenetic analysis based on chloroplast genomes indicates that R. chingii is closely related to Lindenbergia philippensis.