NF-kappaB elements associated with the Stat6 site in the germline gamma1 immunoglobulin promoter are not necessary for the transcriptional response to CD40 ligand.
NF-kappaB elements associated with the Stat6 site in the germline gamma1 immunoglobulin promoter are not necessary for the transcriptional response to CD40 ligand.
复制标题
与种系 gamma1 免疫球蛋白启动子中 Stat6 位点相关的 NF-kappaB 元件对于 CD40 配体的转录反应不是必需的。
DOI：
10.1093/intimm/dxh175
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Dunnick,WesleyA
中科院分区：
文献类型：
--
作者：
Berton,MichaelT;Linehan,LeslieA;Wick,KerilynR;Dunnick,WesleyA
Both germline transcription and switch recombination of heavy chain genes are likely to be regulated byciselements binding transcription factors in the promoter regions of germline immunoglobulin genes. To identifycis-acting elements important in germline transcription of the murine γ1 heavy chain gene, we have used a transgenic approach. Seventeen kb γ1 immunoglobulin transgenes with mutations in three NF-κB sites in the γ1 proximal promoter, a putative CD40 response element, are expressed well. Compared to wild-type transgenes, there is no deficiency in the expression of the transgenes with mutations of the three NF-κB sites after induction of splenic B cells with IL-4 alone, CD40L, or CD40L + IL-4. There may be a small reduction in the response of these mutant transgenes after induction with LPS + IL-4. We also prepared transgenes that were truncated at −150 (rather than −2100) and therefore included the wild-type Stat6 binding site at −123 and the three wild-type NF-κB sites. Nevertheless, γ1 germline transcripts were not expressed from these transgenes. We conclude that the three proximal NF-κB sites are dispensable for expression of γ1 germline transcripts under most conditions. However,cis-acting elements distal to −150 must be critical to this transcription.
