Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.
Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for Clostridium botulinum group I strains.
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DOI：
10.1016/j.fm.2016.06.003
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发表时间：
2016-10
影响因子：
5.3
通讯作者：
Bullough PA
中科院分区：
文献类型：
--
作者：
Janganan TK;Mullin N;Tzokov SB;Stringer S;Fagan RP;Hobbs JK;Moir A;Bullough PA
产孢梭菌是一种非致病性的近亲，也是I组（蛋白水解）神经毒素产生型肉毒梭菌菌株的替代物。外孢壁，这些物种孢子的囊状最外层，可能有助于粘附，传播和毒性。在孢子外壁上发现了一个次晶阵列、毛状绒毛和几个附属物。产孢菌株NCIMB 701792的电镜和原子力显微镜观察。纯化的外孢壁的蛋白质组成通过来自主要单独SDS-PAGE分离的蛋白质条带和来自散装外孢壁的胰蛋白酶肽的LC-MS/MS进行探索。两个高分子量蛋白条带均含有具有胶原样重复结构域的相同蛋白，其可能是毛状绒毛的成分，以及富含半胱氨酸的蛋白CsxA和CsxB。还鉴定了第三种富含半胱氨酸的蛋白（CsxC）。这三种蛋白质也在C.肉毒杆菌Prefecture 594和同源物（75-100%氨基酸同一性）在许多其它I组菌株中编码。这项工作提供了第一次深入了解可能的组成和组织的外孢壁组I C。肉毒杆菌孢子生孢梭菌的孢子有一个类晶状的外表面（孢子外壁）。孢子表面饰有许多丝状物和附属物。纤维包括功能未知的长珠状细丝。存在具有胶原样重复结构域的BclA样外孢壁蛋白。新的富含半胱氨酸的外孢壁蛋白可能构成外孢壁基底阵列。
Clostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75–100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores. Clostridium sporogenes spores have a paracrystalline outer surface (exosporium). The spore surface is decorated with a variety of filaments and appendages. Appendages include long beaded filaments of unknown function. BclA-like exosporium proteins with collagen-like repeat domains are present. Novel cysteine-rich exosporium proteins may compose the exosporium basal array.