Quaternized chitosans bind onto preexisting biofilms and eradicate pre-attached microorganisms.
Quaternized chitosans bind onto preexisting biofilms and eradicate pre-attached microorganisms.
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Quaternized壳聚糖结合了先前存在的生物膜，并消除了预先附着的微生物。
DOI：
10.1039/c4tb01131g
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发表时间：
2014-12-28
期刊：
影响因子：
--
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Jiang F;Deng Y;Yeh CK;Sun Y
以碘甲烷和壳聚糖为原料，合成了不同季铵化程度的N，N，N-三甲基壳聚糖（TMC）。通过FT-IR和1H-NMR表征证实了该反应。抑菌试验表明，该TMC对革兰氏阳性菌表皮葡萄球菌、革兰氏阴性菌大肠杆菌和酵母菌白色念珠菌具有较强的杀菌作用。在聚甲基丙烯酸甲酯（PMMA）膜上形成细菌和真菌生物膜，然后用TMC水溶液处理。Zeta电位的测定表明TMC结合到预先存在的生物膜。以苯基标记的TMC为模型化合物，研究了季铵化壳聚糖与生物膜的结合动力学。集落形成单位（CFU）测定和SEM、共聚焦激光扫描显微镜（CLSM）和荧光显微镜研究表明，键合的TMC具有强大的杀生物活性，以根除预先附着在预先存在的生物膜中的细菌和真菌细胞。MTT法检测TMC与大鼠皮肤成纤维细胞的生物相容性。
Quaternized chitosans, N,N,N-trimethylchitosans (TMC) with different degree of quaternization were synthesized by reacting methyl iodide with chitosan. The reaction was confirmed by FT-IR and 1H-NMR characterization. Antimicrobial assay showed that the prepared TMC had potent biocidal effects against planktonic Gram-positive bacteria Staphylococcus epidermidis, Gram-negative bacteria Escherichia coli, and yeast Candida albicans. Bacterial and fungal biofilms were formed on poly(methyl methacrylate) (PMMA) films and then treated with TMC aqueous solutions. Zeta potential measurement suggested that TMC bonded onto the preexisting biofilms. Biofilm-binding kinetics was evaluated in UV studies using phenyl group-labeled TMC as model compounds, which revealed that quaternized chitosans bonded onto the preexisting biofilms rapidly. Colony-forming unit (CFU) determination and SEM, confocal laser scanning microscopy (CLSM) and fluorescence microscopy studies demonstrated that the bonded TMC had powerful biocidal activities to eradicate the pre-attached bacterial and fungal cells in the preexisting biofilms. The biocompatibility of the TMC samples with rat skin fibroblast cells was evaluated in the MTT assay.