Identification of novel transcription-regulating genes expressed during murine molar development
Identification of novel transcription-regulating genes expressed during murine molar development
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DOI：
10.1002/dvdy.23808
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发表时间：
2012-07-01
影响因子：
2.5
通讯作者：
Asahara, Hiroshi
中科院分区：
文献类型：
--
作者：
Uchibe, Kenta;Shimizu, Hirohito;Asahara, Hiroshi
背景资料：牙齿发育的机制是一个复杂的过程，受到许多基因的调控，包括转录因子、生长因子和其他细胞内和细胞外分子。特别是，转录因子在基因表达中发挥核心作用，调节包括器官发生在内的广泛的生物过程。大量使用基因工程动物模型的研究已经证明了这一点。然而，牙齿发育的详细分子机制尚未完全阐明，部分原因是许多在牙齿发育中起重要作用的基因尚未确定。结果：在这项研究中，我们进行了基于表达的筛选基因表达数据库和原位杂交检测。基于基因表达数据库EMBRYS，1,520个基因中有207个在上颌骨和/或下颌骨中表达，因此被选择用于通过切片原位杂交进行进一步分析。在这些候选人中，28个基因被新确定为与牙齿发育相关的潜在因素，通过原位杂交测定与胚胎第13.5和14.5天的小鼠胚胎的额叶切片。在胚胎16.5和18.5天的表达模式也进行了检查。结论：这些结果将有助于阐明牙齿发育的机制和促进牙齿再生技术的发展。发展动力学241：12171226，2012年。(c)2012 Wiley Periodicals，Inc.
Background: The mechanism of tooth development is a complex process regulated by numerous genes including transcription factors, growth factors, and other intra- and extracellular molecules. Especially, transcription factors play a central role in gene expression, regulating a wide spectrum of biological processes including organogenesis. Substantial evidence has been demonstrated by a number of studies using genetically engineered animal models. However, detailed molecular mechanisms of tooth development have not been completely elucidated, partially because numerous genes that play essential roles in tooth development remain unidentified. Results: In this study, we conducted an expression-based screening using gene expression database and in situ hybridization assays. Based on the gene expression database EMBRYS, 207 out of 1,520 genes were expressed in the maxillary and/or mandibular processes and thus were selected for further analysis by section in situ hybridization. Among these candidates, 28 genes were newly identified as potential factors associated with tooth development by in situ hybridization assays with frontal sections of embryonic day 13.5 and 14.5 mouse embryos. The expression patterns were also examined at embryonic day 16.5 and 18.5. Conclusions: These results will contribute to elucidating the mechanisms of tooth development and to improving the technology for regeneration of tooth. Developmental Dynamics 241:12171226, 2012. (c) 2012 Wiley Periodicals, Inc.