PNA lectin for purifying mouse acinar cells from the inflamed pancreas.
PNA lectin for purifying mouse acinar cells from the inflamed pancreas.
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DOI：
10.1038/srep21127
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发表时间：
2016-02-17
影响因子：
4.6
通讯作者：
Gittes GK
中科院分区：
文献类型：
--
作者：
Xiao X;Fischbach S;Fusco J;Zimmerman R;Song Z;Nebres P;Ricks DM;Prasadan K;Shiota C;Husain SZ;Gittes GK
需要更好的方法来纯化人类或小鼠腺泡细胞，而不需要进行基因修饰。此类技术将有利于某些机制的具体研究，例如腺泡到β细胞重编程和胰腺炎。 Ulex Europaeus 凝集素 I (UEA-I) 凝集素已用于标记和分离胰腺中的腺泡细胞。然而，UEA-I 阳性细胞级分的纯度尚未得到充分评估。在这里，我们筛选了 20 种广泛使用的凝集素对主要胰腺细胞类型的结合特异性，发现 UEA-I 和花生凝集素 (PNA) 对小鼠胰腺中的腺泡细胞具有特异性亲和力，而对其他主要胰腺细胞类型（包括内分泌细胞、导管细胞和内皮细胞）具有最小的亲和力。此外，与UEA-I纯化的腺泡细胞相比，PNA纯化的腺泡细胞受到间充质和炎症细胞的污染较少。因此，UEA-I和PNA似乎是用于纯化胰腺腺泡细胞的优良凝集素。在胰腺中间充质细胞或炎症细胞显着增加的情况下，例如1型糖尿病、胰腺炎和胰腺癌，PNA可能是更好的选择。
Better methods for purifying human or mouse acinar cells without the need for genetic modification are needed. Such techniques would be advantageous for the specific study of certain mechanisms, such as acinar-to-beta-cell reprogramming and pancreatitis. Ulex Europaeus Agglutinin I (UEA-I) lectin has been used to label and isolate acinar cells from the pancreas. However, the purity of the UEA-I-positive cell fraction has not been fully evaluated. Here, we screened 20 widely used lectins for their binding specificity for major pancreatic cell types, and found that UEA-I and Peanut agglutinin (PNA) have a specific affinity for acinar cells in the mouse pancreas, with minimal affinity for other major pancreatic cell types including endocrine cells, duct cells and endothelial cells. Moreover, PNA-purified acinar cells were less contaminated with mesenchymal and inflammatory cells, compared to UEA-I purified acinar cells. Thus, UEA-I and PNA appear to be excellent lectins for pancreatic acinar cell purification. PNA may be a better choice in situations where mesenchymal cells or inflammatory cells are significantly increased in the pancreas, such as type 1 diabetes, pancreatitis and pancreatic cancer.