BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner.
BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner.
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发表时间：
1996-07
期刊：
影响因子：
11.2
通讯作者：
Yumay Chen;A. A. Farmer-A.;Chi‐Fen Chen;Diane C. Jones;Phang-lang Chen;Wen-Hwa Lee
中科院分区：
文献类型：
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作者：
Yumay Chen;A. A. Farmer-A.;Chi‐Fen Chen;Diane C. Jones;Phang-lang Chen;Wen-Hwa Lee
小鼠多克隆抗体，提出了对三个地区的人BRCA1蛋白，其特征在于，揭示BRCA1作为一个220 kDa的核磷蛋白在正常细胞。所有三种抗血清均识别体外翻译的和重组的杆状病毒衍生的BRCA 1，其与来自人乳腺上皮细胞系HBL100的BRCA 1共迁移。BRCA1的表达和磷酸化被证明是细胞周期依赖性的，最大的表达和磷酸化发生在S和M期。细胞周期蛋白依赖性激酶2和其他与细胞周期蛋白D和A相关的激酶被证明与BRCA 1结合并磷酸化，这表明BRCA 1的生物活性可能受到细胞周期蛋白依赖性激酶的调节。
Mouse polyclonal antibodies, raised against three regions of the human BRCA1 protein, were characterized and revealed BRCA1 as a 220-kDa nuclear phosphoprotein in normal cells. All three antisera recognize both in vitro-translated and recombinant, baculovirus-derived BRCA1, which co-migrate with BRCA1 from the human breast epithelia cell line, HBL100. BRCA1 expression and phosphorylation are shown to be cell cycle dependent, with greatest expression and phosphorylation occurring in S and M phases. Cyclin-dependent kinase 2 and other kinases associated with cyclins D and A are shown to bind to and phosphorylate BRCA1, suggesting that the biological activity of BRCA1 may be regulated by cyclin-dependent kinases.