CHEMOANATOMICAL ORGANIZATION OF THE NORADRENERGIC INPUT FROM LOCUS COERULEUS TO THE OLFACTORY-BULB OF THE ADULT-RAT
CHEMOANATOMICAL ORGANIZATION OF THE NORADRENERGIC INPUT FROM LOCUS COERULEUS TO THE OLFACTORY-BULB OF THE ADULT-RAT
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DOI：
10.1002/cne.902850305
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发表时间：
1989-07-15
影响因子：
2.5
通讯作者：
REYHER, CKH
中科院分区：
文献类型：
--
作者：
MCLEAN, JH;SHIPLEY, MT;REYHER, CKH
蓝斑含有广泛投射到中枢神经系统的去甲肾上腺素能神经元。大鼠蓝斑投射的主要目标是嗅球（Shipley等人：Brain Res. 329：294-299，'' 85），但球内投射的组织尚未被系统地检查。本研究应用顺行追踪和免疫细胞化学技术，对主嗅球和副嗅球内蓝斑去甲肾上腺素能纤维的层分布和密度进行了研究。将1%麦胚凝集素-辣根过氧化物酶离子导入蓝斑后，在副嗅球的外丛状层、颗粒细胞层，特别是在僧帽细胞层的内部观察到顺向标记。在肾小球层中几乎没有观察到标记。在平均嗅球，标记的轴突中观察到的颗粒细胞层，在内部和外部丛状层，偶尔在二尖瓣细胞层，和最不经常在肾小球层。嗅球中的去甲肾上腺素能纤维通过使用多巴胺-β-受体抗体的免疫细胞化学来鉴定。羟化酶去甲肾上腺素能神经支配的层模式和密度用定量图像分析确定。在副嗅球中，致密神经支配位于二尖瓣细胞层的最内部，其次是颗粒细胞层、二尖瓣细胞层的表面部分和外部丛状层。肾小球层纤维密度最小。去甲肾上腺素能纤维在主嗅球和副嗅球内的层状分布模式相似。目前的研究表明，蓝斑去甲肾上腺素能纤维终止优先在内部网状，颗粒细胞，和外部网状层。这表明蓝斑输入到主嗅球和副嗅球的主要影响是在这些层中占主导地位的神经元元件，颗粒细胞。需要更多的研究来解决这种输入如何影响特定的嗅球回路。
The locus coeruleus contains noradrenergic neurons which project widely throughout the CNS. A major target of locus coeruleus projections in the rat is the olfactory bulb (Shipley et al.: Brain Res. 329:294-299, ''85) but the organization of the projections within the bulb has not been systematically examined. In this study, the laminar distribution and densities of locus coeruleus-noradrenergic fibers in the main and accessory olfactory bulbs were determined with anterograde tracing and immunocytochemical techniques. Following iontophoretic injections of 1% wheat germ agglutinin-horseradish peroxidase into the locus coeruleus, the densest anterograde label in the accessory olfactory bulb was observed in the external plexiform layer, granule cell layer, and especially in the internal part of the mitral cell layer. Virtually no label was observed in the glomerular layer. In the mean olfactory bulb, labelled axons were observed in the granule cell layer, in the internal and external plexiform layers, occasionally in the mitral cell layer, and least often in the glomerular layer. Noradrenergic fibers in the olfactory bulb were identified by using immunocytochemistry with an antibody to dopamine-.beta.-hydroxylase. Laminar patterns and densities of noradrenergic innervation were determined with quantitative image analysis. In the accessory olfactory bulb, the densest innervation was in the innermost portion of the mitral cell layer followed by the granule cell layer, the superficial part of the mitral cell layer, and the external plexiform layer. The density of fibers in the glomerular layer was least. The laminar pattern of noradrenergic fiber distribution in the main olfactory bulb was similar to that in accessory olfactory bulb. The present studies demonstrate that locus coeruleus-noradrenergic fibers terminate preferentially in the internal plexiform, granule cell, and external plexiform layers. This suggests that the major influence of the locus coeruleus input to both the main and accessory the olfactory bulbs is on the predominant neuronal element in those layers, the granule cells. Additional studies are needed to resolve how this input influences specific olfactory bulb circuits.