Evidence that specific oligosaccharides block early events necessary for the expression of antigen-specific proliferation by human lymphocytes.
Evidence that specific oligosaccharides block early events necessary for the expression of antigen-specific proliferation by human lymphocytes.
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有证据表明，特定寡糖可阻断人类淋巴细胞表达抗原特异性增殖所需的早期事件。
DOI：
10.4049/jimmunol.125.3.1306
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发表时间：
1980
影响因子：
4.4
通讯作者：
R. Blaese
中科院分区：
文献类型：
--
作者：
A. Muchmore;J. Decker;R. Blaese
我们以前已经表明，单糖和二糖将阻断自发单核细胞介导的细胞毒性的表达。我们的数据是一致的假设，即人单核细胞表达凝集素样受体，能够结合到靶细胞膜上发现的各种糖部分。在这篇文章中，我们将提出证据表明单糖和二糖也能够阻断T细胞反应性的表达，如通过体外抗原特异性增殖测定所测量的。阻断单核细胞介导的细胞毒性的大多数糖对抗原特异性增殖没有影响。那些抑制抗原诱导的增殖的糖对PHA诱导的增殖没有影响。此外，这些糖中的一些仅在测定开始时加入才有抑制作用;如果在测定开始后24小时加入，则不能抑制。抗原脉冲实验表明，这些糖不阻断抗原摄取的人单核细胞。这些数据表明，各种细胞相互作用可能是由单糖特异性受体介导的。在体外和体内特异性阻断这些天然存在的凝集素的能力可能被证明是解剖出各种形式的细胞识别和协作的有力工具。
We have previously shown that monosaccharides and disaccharides will block the expression of spontneous monocyte-mediated cytotoxicity. Our data were consistent with the hypothesis that human mononuclear cells express lectin-like receptors that are capable of binding to a variety sugar moieties found on target cell membranes. In this communication, we will present evidence that monosaccharides and disaccharides are also capable of blocking the expression of T cell reactivity as measured by an in vitro antigen-specific proliferative assay. The majority of sugars that blocked monocyte-mediated cytotoxicity had no effect on antigen-specific proliferation. Those sugars that did suppress antigen-induced proliferation had no effect on PHA-induced proliferation. Furthermore, some of these sugars only inhibited if they were added at the initiation of the assay; they failed to inhibit if added 24 hr after the initiation of the assay. Antigen-pulsing experiments suggested that these sugars did not block antigen uptake by human monocytes. These data suggest that a variety of cellular interactions may be mediated by receptors with specificity for simple sugars. The ability to block these naturally occurring lectins specifically both in vitro and in vivo may prove to be a powerful tool for dissecting out various forms of cellular recognition and collaboration.