Tunable Silver-Containing Stationary Phases for Multidimensional Gas Chromatography
Tunable Silver-Containing Stationary Phases for Multidimensional Gas Chromatography
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用于多维气相色谱的可调含银固定相
DOI：
10.1021/acs.analchem.9b00472
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发表时间：
2019
影响因子：
7.4
通讯作者：
Anderson, Jared L.
中科院分区：
文献类型：
--
作者：
Souza, Israel D.;Nan, He;Queiroz, Maria Eugênia;Anderson, Jared L.
为了实现多维气相色谱（MDGC）对复杂样品的高分离能力，所用固定相的选择性至关重要。非极性×极性色谱柱组合仍然是MDGC中最常用的色谱柱组合。然而，含有具有非常相似的性质的轻分析物的混合物的分辨率仍然是一个巨大的挑战。提供独特分离机制的固定相的开发有可能显着改善MDGC分离，特别是在解决复杂样品中的共洗脱峰。首次设计了一种含银（I）离子的固定相，并将其用作二维气相色谱（GC × GC），用于含炔、二烯、萜、酯、醛和酮的混合物的分离。与广泛使用的非极性和极性柱组相比，银基柱通过提供更好的共洗脱化合物的色谱分离而表现出优越的上级性能。采用GC × GC方法成功地分离了不饱和脂肪酸的混合物，该方法在第二维的洗脱顺序高度依赖于分析物中双键的数目。
To achieve high separation power of complex samples using multidimensional gas chromatography (MDGC), the selectivity of the employed stationary phases is crucial. The nonpolar × polar column combination remains the most popular column set used in MDGC. However, resolution of mixtures containing light analytes possessing very similar properties remains a formidable challenge. The development of stationary phases that offer unique separation mechanisms have the potential to significantly improve MDGC separations, particularly in resolving coeluting peaks in complex samples. For the first time, a stationary phase containing silver(I) ions was successfully designed and employed as a second-dimension column using comprehensive two-dimensional gas chromatography (GC × GC) for the separation of mixtures containing alkynes, dienes, terpenes, esters, aldehydes, and ketones. Compared with a widely used nonpolar and polar column set, the silver-based column exhibited superior performance by providing better chromatographic resolution of coeluting compounds. A mixture of unsaturated fatty acids was successfully separated using a GC × GC method in which the elution order in the second dimension was highly dependent on the number of double bonds within the analytes.