Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin-nicotinamide induced diabetic rats
Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin-nicotinamide induced diabetic rats
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DOI：
10.1016/j.lfs.2009.10.021
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发表时间：
2009-12-16
期刊：
影响因子：
6.1
通讯作者：
Sankaranarayanan, Chandrasekaran
中科院分区：
文献类型：
--
作者：
Pari, Leelavinothan;Sankaranarayanan, Chandrasekaran
目的：本研究旨在评价黑种草子主要成分百里醌（thymoquinone，TQ）对链脲佐菌素（streptozotocin，STZ）-烟酰胺（nicotinamide，NA）诱导的糖尿病大鼠糖代谢关键酶活性的降血糖作用。在体重180-220 g的实验大鼠中，通过单次腹腔注射（i.p）注射STZ（45 mg/kg B.w），在i.p给予NA（110 mg/kg B.w）后15 min。糖尿病大鼠灌胃TQ 20、40、80 mg/kg B.w，连续45天。检测血糖、胰岛素、糖化血红蛋白（HbA（1C））、血红蛋白（Hb）水平。己糖激酶的活性。结果：TQ连续口服45天，可剂量依赖性地改善STZ-NA诱导的糖尿病大鼠的血糖状态。胰岛素、血红蛋白水平升高，血糖和HbA（1C）水平显著降低。改变的碳水化合物代谢酶的活性恢复到接近正常。TQ.Significance：这些结果表明，TQ在80 mg/kg B.w与肝酶活性的有益变化相关，从而发挥潜在的抗高血糖作用。(C)2009爱思唯尔公司All rights reserved.
Aims: The present study was designed to evaluate the antihyperglycemic potential of thymoquinone (TQ), major constituent of Nigella sativa seeds on the activities of key enzymes of carbohydrate metabolism in streptozotocin (STZ)-nicotinamide (NA)-induced diabetic rats.Main methods: Diabetes was induced in experimental rats weighing 180-220 g, by a single intraperitoneal (i.p) injection of STZ (45 mg/kg b.w), 15 min after the i.p administration of NA (110 mg/kg b.w). Diabetic rats were administered TQ intragastrically at 20, 40, 80 mg/kg b.w for 45 days. The levels of plasma glucose, insulin, glycated hemoglobin (HbA(1C)) and hemoglobin (Hb) were measured. The activities of hexokinase. glucose 6-phosphate dehydrogenase, glucose 6-phosphatase and fructose 1,6-bisphosphatase were assayed in liver homogenates.Key findings: Oral administration of TQ for 45 days, dose dependently improved the glycemic status in STZ-NA induced diabetic rats. The levels of insulin, Hb increased with significant decrease in glucose and HbA(1C) levels. The altered activities of carbohydrate metabolic enzymes were restored to near normal. No significant changes were noticed in normal rats treated with TQ.Significance: These results show that TQ at 80 mg/kg b.w is associated with beneficial changes in hepatic enzyme activities and thereby exerts potential anti hyperglycemic effects. (C) 2009 Elsevier Inc. All rights reserved.