Vectors that facilitate the replacement of transcriptional lacZ fusions in Streptococcus mutans and Bacillus subtilis with fusions to gfp or gusA.
Vectors that facilitate the replacement of transcriptional lacZ fusions in Streptococcus mutans and Bacillus subtilis with fusions to gfp or gusA.
复制标题
有助于将变形链球菌和枯草芽孢杆菌中的转录 lacZ 融合体替换为 gfp 或 gusA 融合体的载体。
DOI：
10.1016/j.femsle.2005.05.001
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Piggot,PatrickJ
中科院分区：
文献类型：
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作者：
Chary,VasantK;Busuioc,Monica;RenyeJr,JohnA;Piggot,PatrickJ
已经构建了变形链球菌和枯草芽孢杆菌的质粒载体，这些质粒载体可以快速地用gfp（编码绿色荧光蛋白）和orgusA（编码β-葡萄糖醛酸酶）替换广泛使用的报告基因lacZ（编码β-半乳糖苷酶）。lacZ → gf前置载体极大地促进了S.变种和产孢B.枯草芽孢杆菌ThelacZ→ gusA置换载体便于比较同一生物体中的两种启动子。还描述了使gusA能够被gfpinB替换的载体。枯草杆菌。
Plasmid vectors have been constructed forStreptococcus mutansandBacillus subtilisthat make possible rapid replacement of the widely used reporter genelacZ(encoding β-galactosidase) with eithergfp(encoding green fluorescent protein) orgusA(encoding β-glucuronidase). ThelacZ→gfpreplacement vectors greatly facilitate the analysis of the spatial location of gene expression in biofilms ofS. mutansand in sporulatingB. subtilis. ThelacZ→gusAreplacement vectors facilitate the comparison of two promoters within the same organism. A vector is also described that enablesgusAto be replaced withgfpinB. subtilis.