Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
Transgenic Lettuce Producing a Candidate Protein for Vaccine against Edema Disease
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DOI：
10.1271/bbb.90129
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发表时间：
2009-07-01
影响因子：
1.6
通讯作者：
Kato, Ko
中科院分区：
文献类型：
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作者：
Matsui, Takeshi;Asao, Hiroshi;Kato, Ko
猪水肿病是由产滋贺毒素2 e的大肠杆菌引起的细菌性疾病，主要属于血清型O 138、O 139和O 141。滋贺毒素2 e的B亚基（Stx 2 e B）是用于针对水肿疾病的疫苗的候选蛋白质。我们在转基因莴苣（Lactucasativa）中生产了这种蛋白质，这是一种可以在工厂环境中种植的可食用植物。在瞬时表达系统中，我们发现NtADH 5 '-非翻译区（5'-UTR）在莴苣细胞中起翻译增强子的作用，并且Stx 2 eB在莴苣细胞的内质网（ER）中最有效地积累。在稳定的转基因莴苣植物中产生Stx 2 eB，所述稳定的转基因莴苣植物表达与NtADH 5 '-UTR和编码ER定位信号的序列融合的经修饰的Stx 2 eB基因。
Pig edema disease is a bacterial disease caused by Shiga toxin 2e-producing Escherichia coli belonging mainly to serotypes O138, O139, and O141. The B subunit of Shiga toxin 2e (Stx2eB) is a candidate protein for use in a vaccine against edema disease. We produced this protein in transgenic lettuce (Lactuca sativa), an edible plant that can be cultivated in a factory setting. In a transient expression system, we found that NtADH 5'-untranslated region (5'-UTR) functions as a translational enhancer in lettuce cells, and that Stx2eB accumulates most efficiently in the endoplasmic reticulum (ER) of lettuce cells. Stx2eB was produced in stable transgenic lettuce plants expressing a modified Stx2eB gene fused with the NtADH 5'-UTR and sequence encoding ER localization signals.