Influence of the murine oestrous cycle on the induction of mucosal immunity
Influence of the murine oestrous cycle on the induction of mucosal immunity
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DOI：
10.1034/j.1600-0897.2003.00097.x
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发表时间：
2003-11-01
影响因子：
3.6
通讯作者：
Beagley, KW
中科院分区：
文献类型：
--
作者：
Gockel, CM;Bao, SS;Beagley, KW
问题：要确定是否发情周期的阶段，在免疫的时候，影响粘膜和全身immunity. Method的大小研究：雌性BALB/c小鼠免疫破伤风类毒素和霍乱毒素的口服，鼻内和经皮途径。在发情前期、发情期、发情后期和间情期免疫各组小鼠。ELISA法测定血清和粘膜分泌物中的抗体，淋巴细胞增殖试验测定T细胞反应。结果：在周期的雌二醇优势阶段（发情期和发情前期）口服免疫显著增强女性生殖道（FRT）分泌物中TT特异性IgG和伊加水平和粪便提取物中TT特异性伊加水平。经皮免疫在动情间期增强粪便提取物中的TT特异性IgG。TT-特异性T细胞增殖是最大的鼻内免疫在发情前期和经皮免疫在发情间期，特别是在尾部和腰部淋巴结引流FRT和colon.CONCLUSIONS：生殖周期相关的内源性激素雌二醇和孕酮的变化影响疫苗诱导的免疫水平在FRT和远端结肠口服和经皮免疫接种。
PROBLEM: To determine if the stage of oestrous cycle, at the time of immunization, affects the magnitude of mucosal and systemic immunity.METHOD OF STUDY: Female BALB/c mice were immunized with tetanus toxoid and cholera toxin by the oral, intranasal and transcutaneous routes. Groups of mice were immunized at proestrus, oestrus, postestrus and diestrus. Antibodies in serum and mucosal secretions were determined by ELISA and T cell responses by lymphocyte proliferation assay.RESULTS: Oral immunization at the oestradiol dominant stage of cycle (oestrus and proestrus) significantly enhanced TT-specific IgG and IgA levels in female reproductive tract (FRT) secretions and TT-specific IgA levels in faecal extracts. Transcutaneous immunization at diestrus enhanced TT-specific IgG in faecal extracts. TT-specific T cell proliferation is greatest following intranasal immunization at proestrus and transcutaneous immunization at diestrus, particularly in the caudal and lumbar lymph nodes draining the FRT and colon.CONCLUSIONS: Reproductive cycle-associated changes in the endogenous sex hormones oestradiol and progesterone influence the levels of vaccine-induced immunity in the FRT and distal colon following oral and transcutaneous immunization.