BODY-WALL MUSCLE FORMATION IN CAENORHABDITIS-ELEGANS EMBRYOS THAT LACK THE MYOD HOMOLOG HLH-1
BODY-WALL MUSCLE FORMATION IN CAENORHABDITIS-ELEGANS EMBRYOS THAT LACK THE MYOD HOMOLOG HLH-1
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DOI：
10.1126/science.1314423
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发表时间：
1992-04-10
期刊：
影响因子：
56.9
通讯作者：
FIRE, A
中科院分区：
文献类型：
--
作者：
CHEN, L;KRAUSE, M;FIRE, A
DNA 结合蛋白 myoD 家族与多种生物体的肌生成控制有关。对线虫秀丽隐杆线虫中的同源物的搜索仅产生了一种基因，命名为hlh-1，在体壁肌肉细胞及其前体细胞中表达。为了评估 hlh-1 在秀丽隐杆线虫肌发生中的作用，在伽马射线照射后分离出跨越 hlh-1 位点的遗传缺陷。这些缺陷的纯合胚胎表现出广泛的体壁肌肉分化，包括几种特征性肌丝蛋白的表达和弱收缩行为。因此，体壁肌肉前体细胞不需要合子 hlh-1 表达来适应肌细胞的命运。
The myoD family of DNA binding proteins has been implicated in the control of myogenesis in a variety of organisms. Searches for homologs in the nematode Caenorhabditis elegans yielded only one gene, designated hlh-1, expressed in body-wall muscle cells and their precursors. To assess the role of hlh-1 in C. elegans myogenesis, genetic deficiencies spanning the hlh-1 locus were isolated after gamma irradiation. Embryos homozygous for these deficiencies exhibited extensive body-wall muscle differentiation, including expression of several characteristic myofilament proteins and weak contracile behavior. Thus, zygotic hlh-1 expression was not required for body-wall muscle precursors to adopt muscle cell fates.