Comprehensive genomic analysis of cell wall genes in Aspergillus nidulans
Comprehensive genomic analysis of cell wall genes in Aspergillus nidulans
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DOI：
10.1016/j.fgb.2008.07.022
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发表时间：
2009-03-01
影响因子：
3
通讯作者：
Klis, Frans M.
中科院分区：
文献类型：
--
作者：
de Groot, Piet W. J.;Brandt, Bernd W.;Klis, Frans M.
了解曲霉细胞壁生物合成的机制。与医药和食品安全以及生物技术应用高度相关。构巢曲霉的细胞壁由半乳甘露糖蛋白、1，3-α-葡聚糖、β-葡聚糖和几丁质组成。在这里，我们提出了一个全面的库存的细胞壁相关基因在A。nidulans。这包括聚糖合成和聚糖加工酶、孢子壁成熟酶、GPI锚加工酶、GPI蛋白和疏水蛋白以及细胞壁完整性途径的信号蛋白。所有主要的已知真菌细胞壁相关基因的代表，除了Pir-CWP。重要的是，我们已经确定了可能参与1，3/1，4-β-葡聚糖合成的基因产物，并且我们提出四种预测的GPI蛋白，一种混合连接的β-葡聚糖酶和三种淀粉酶样α-葡聚糖酶，可能分别具有与1，3/1，4-β-葡聚糖和1，3/1，4-α-葡聚糖加工有关的转糖苷活性。我们进一步提出了一个更新的调查推定GPI蛋白。最后，我们提出了质谱证据表明，存在至少12个共价连接的细胞壁蛋白在菌丝壁的A。包括10种预测的GPI蛋白，其中大多数属于碳水化合物活性酶家族，其也在子囊菌酵母的壁中发现。(C)2008年爱思唯尔公司All rights reserved.
Knowledge of the mechanisms underlying cell wall biosynthesis in Aspergillus spp. is of high relevance to medicine and food safety, and for biotechnological applications. The cell wall of Aspergillus nidulans is composed of galactomannoproteins, 1,3-alpha-glucan, beta-glucans, and chitin. Here, we present a comprehensive inventory of the cell wall-related genes in A. nidulans. This includes glycan-synthetic and glycan-processing enzymes, Spore wall maturation enzymes, GPI-anchor processing enzymes, GPI proteins and hydrophobins, and signaling proteins of the cell wall integrity pathway. All major known fungal cell wall-related genes are represented, except for Pir-CWPs. Importantly, we have identified a gene product that is possibly involved in the synthesis of 1,3/1,4-beta-glucan, and we propose that four predicted GPI proteins, a mixed-linked beta-glucanase and three amylase-like alpha-glucanases, may have transglucosidic activities pertaining to the processing of 1,3/1,4-beta-glucan and 1,3/1,4-alpha-glucan, respectively. We further present an updated survey of putative GPI proteins. Finally, we present mass spectrometric evidence suggesting the presence of at least twelve covalently-linked cell wall proteins in the hyphal wall of A. nidulans, including ten predicted GPI proteins, most of which belong to carbohydrate-active enzyme families that are also found in the walls of ascomycetous yeasts. (C) 2008 Elsevier Inc. All rights reserved.