PROJECTIONS OF THE SUPRACHIASMATIC NUCLEUS TO STRESS-RELATED AREAS IN THE RAT HYPOTHALAMUS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
PROJECTIONS OF THE SUPRACHIASMATIC NUCLEUS TO STRESS-RELATED AREAS IN THE RAT HYPOTHALAMUS - A LIGHT AND ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1002/cne.903350104
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发表时间：
1993-09-01
影响因子：
2.5
通讯作者：
SHINN, S
中科院分区：
文献类型：
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作者：
BUIJS, RM;MARKMAN, M;SHINN, S
本研究的目的是研究下丘脑中视交叉上核（SCN）可能影响皮质类固醇分泌的部位。尽管已经确定了scn介导的促肾上腺皮质激素（ACTH）和皮质类固醇分泌的日常节律，但先前确定视交叉上核投射的研究未能说明与促肾上腺皮质激素释放激素神经元（CRH）的直接联系。为了确定中枢神经系统中SCN可能影响皮质类固醇分泌的位置，选择了包含SCN传出信号的区域，这些区域与参与应激反应的神经元接触。为了在本研究中实现这一目标，我们通过Pha-L示踪来观察SCN的输出，并通过c-fos蛋白的免疫细胞化学染色来观察参与应激反应的神经元。通过光镜双染色和电镜免疫细胞化学研究确定了这些传出物接触c-fos阳性神经元的部位。结果表明，除了下丘脑室旁核（PVN）内侧的细胞旁区外，还有更多的区域显示fos阳性神经元。SCN传出信号接触这些神经元的位置仅限于紧邻这些假定的CRH神经元的区域，而不集中在这些神经元本身。这些区域包括心室周围和吻侧PVN以及下丘脑背内侧：已知这三个区域都投射到PVN。因此，我们得出结论，SCN将其与皮质类固醇分泌相关的信息通过PVN内部和周围的中间神经元传递给含crh的神经元，而不是通过与这些神经元本身直接相互作用。(C) 1993 Wiley-Liss, Inc。
The purpose of the present study was to investigate the sites in the hypothalamus where the suprachiasmatic nucleus (SCN) may influence corticosteroid secretion. In spite of the well established, SCN-mediated, daily rhythms in adrenocorticotrophic hormone (ACTH) and corticosteroid secretion, previous studies determining the projections of the suprachiasmatic nucleus failed to illustrate direct connections with corticotrophin-releasing hormone neurons (CRH).In order to identify where in the central nervous system the SCN may influence corticosteroid secretion, areas were selected that contained SCN efferents contacting neurons involved in the stress response. To achieve this in the present study, SCN efferents were visualized by Pha-L tract-tracing, together with the neurons involved in the stress response by immunocytochemical staining for c-fos protein.The sites where these efferents contacted c-fos-positive neurons were established by light microscopic double staining and electron microscopic immunocytochemical studies. It appeared that apart from the medial parvocellular area of the paraventricular nucleus (PVN) of the hypothalamus, many more regions showed fos-positive neurons. Sites where SCN efferents contacted such neurons are limited only to areas immediately adjacent to these putative CRH neurons but are not concentrated on these neurons themselves. These areas consist of the periventricular and rostral PVN together with the dorsomedial hypothalamus: all three regions are known to project into the PVN. Therefore, it is concluded that the SCN transmits its information related to corticosteroid secretion via interneurons in and around the PVN to the CRH-containing neurons, rather than by a direct interaction with these neurons themselves. (C) 1993 Wiley-Liss, Inc.