SPECTRAL AND PHOTOPHYSICAL STUDIES OF BENZO[C]XANTHENE DYES - DUAL EMISSION PH SENSORS
SPECTRAL AND PHOTOPHYSICAL STUDIES OF BENZO[C]XANTHENE DYES - DUAL EMISSION PH SENSORS
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DOI：
10.1016/0003-2697(91)90237-n
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发表时间：
1991-05-01
影响因子：
2.9
通讯作者：
PRENDERGAST, FG
中科院分区：
文献类型：
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作者：
WHITAKER, JE;HAUGLAND, RP;PRENDERGAST, FG
一系列荧光长波长苯并[c]呫吨染料已被表征用于激发和发射比应用中的 pH 测量。这些指示剂的两大类是半萘荧光素 (SNAFL) 和半萘荧光素 (SNARF)，它们分别在 10 位被氧或氮取代。这些探针显示荧光团的质子化和去质子化形式的单独发射。染料可以用氩离子激光器在 488 或 514 nm 处激发。大多数指标的 pKa 值在 7.6 至 7.9 之间。对羧基-SNAFL-1 系统进行了详细的光物理研究，并将激发态质子反应与结构相关的衍生物（例如伞形酮）进行了比较。还制备了透膜酯，例如二乙酸酯和乙酰氧基甲酯。这些指示剂在光谱上与 fura-2 和 indo-1 等钙指示剂具有良好的分辨能力，并且适合同时测定 pH 和 Ca2+ 瞬态。
A series of fluorescent, long-wavelength, benzo[c]xanthene dyes has been characterized for pH measurement in both excitation and emission ratio applications. The two general classes of these indicators are seminaphthofluoresceins (SNAFLs) and seminaphthorhodafluors (SNARFs) which are substituted at the 10-position with oxygen or nitrogen, respectively. These probes show separate emissions from the protonated and deprotonated forms of the fluorophores. The dyes may be excited at 488 or 514 nm with argon ion lasers. Most of the indicators have pKavalues between 7.6 and 7.9. Detailed photophysical studies were conducted on the carboxy-SNAFL-1 system and excited-state prototropic reactions were compared to structurally related derivatives, such as the umbelliferones. Membrane permeant esters, such as diacetates and acetoxymethyl esters have also been prepared. The indicators are spectrally well resolved from calcium indicators such as fura-2 and indo-1 and should be suitable for simultaneous determination of pH and Ca2+transients.