p16INK4a and p15INK4b gene promoter methylation in cervical cancer patients
p16INK4a and p15INK4b gene promoter methylation in cervical cancer patients
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DOI：
10.3892/ol.2012.655
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发表时间：
2012-06-01
期刊：
影响因子：
2.9
通讯作者：
Kaur, Jagdeep
中科院分区：
文献类型：
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作者：
Jha, Abhimanyu Kumar;Nikbakht, Mohsen;Kaur, Jagdeep
本研究旨在探讨宫颈癌组织中p16(INK4a)、p14(ARF)和p15(INK4b)基因的甲基化状态以及高甲基化对这些基因表达的影响。甲基化特异性聚合酶链式反应(MSP)分析p16(INK4a)、p14(ARF)和p15(INK4b)基因甲基化状态，并经测序证实。逆转录-聚合酶链式反应(RT-PCR)检测高甲基化对基因表达的影响。在来自印度北部人群的125例宫颈癌标本中，p16(INK4a)、p14(ARF)和p15(INK4b)基因启动子甲基化的发生率分别为36%、8.8%和11.2%。P16(INK4a)基因甲基化显著高于正常对照组(P
The aim of this study was to determine the methylation status of the p16(INK4a), p14(ARF) and p15(INK4b) genes and the subsequent effect of hypermethylation on the expression of these genes in cervical cancer patients. Methylation-specific PCR (MSP) was performed to analyse the methylation status of p16(INK4a), p14(ARF) and p15(INK4b) genes and was confirmed by sequencing. Reverse transcription PCR (RT-PCR) was carried out to determine changes in the expression of the genes due to hypermethylation. Hypermethylation of the p16(INK4a), p14(ARF) and p15(INK4b) gene promoters was observed in 36, 8.8 and 11.2%, respectively, of 125 cervical cancer samples from a north Indian population. Methylation of p16(INK4a) was significantly (P