Bactericidal activity and cytotoxicity of antibacterial monomer MDPB
Bactericidal activity and cytotoxicity of antibacterial monomer MDPB
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DOI：
10.1016/s0142-9612(98)00247-6
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发表时间：
1999-05-01
期刊：
影响因子：
14
通讯作者：
Ebisu, S
中科院分区：
文献类型：
--
作者：
Imazato, S;Ebi, N;Ebisu, S
研究了新型抗菌单体12-甲基丙烯酰氧基十二烷基溴化吡啶（MDPB）的杀菌特性和细胞毒性。为了评价MDPB对口腔链球菌的杀菌活性，测定了对7种菌种的最小杀菌浓度（MBC）和对变形链球菌的时间杀灭动力学。用[H-3]-胸苷掺入法测定不同浓度MDPB对人牙髓细胞的毒性作用。MDPB对7种链球菌有较强的杀菌活性，MBC值为31.1 ~ 62.5 μ g/ml。时间杀伤测定表明，250 μ g/ml或更高浓度的MDPB具有快速杀伤作用，500 μ g/ml或更高浓度的MDPB在1分钟内可杀死所有细胞。与浓度为10 μ g/ml或更低的MDPB接触时未观察到细胞毒性效应，认为MDPB的毒性与牙科材料中使用的其他单体相似。这些结果表明，MDPB可以有效地纳入牙科树脂基材料，以提供对口腔细菌的杀菌活性。(C)1999 Elsevier Science Ltd.保留所有权利。
The aim of this study was to investigate bactericidal characteristics and cytotoxicity of the newly developed antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB). To evaluate the bactericidal activity of MDPB against oral streptococci, the minimum bactericidal concentration (MBC) for seven species and time-kill kinetics against Streptococcus mutans were determined. The cytotoxic effects of MDPB on human pulpal cells were assessed by [H-3]-thymidine uptake after contact with MDPB solutions at various concentrations. MDPB showed strong bactericidal activity against seven streptococci, the MBC value ranging from 31.1 to 62.5 mu g ml(-1). Time-kill determination indicated a rapid killing effect of MDPB at 250 mu g ml(-1) or over, and all cells were billed within 1 min by MDPB at 500 mu g ml(-1) or over. No cytotoxic effect was observed on contact with MDPB at concentrations of 10 mu g ml(-1) or less, and the toxicity of MDPB was considered to be similar to those of other monomers used for dental materials. These results suggest that MDPB can be effectively incorporated in dental resin-based materials to provide bactericidal activity against oral bacteria. (C) 1999 Elsevier Science Ltd. All rights reserved.