Inteins, valuable genetic elements in molecular biology and biotechnology.
Inteins, valuable genetic elements in molecular biology and biotechnology.
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DOI：
10.1007/s00253-010-2628-x
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发表时间：
2010-06
影响因子：
5
通讯作者：
Poeggeler, Stefanie
中科院分区：
文献类型：
--
作者：
Elleuche, Skander;Poeggeler, Stefanie
内链蛋白是一种蛋白质内部元件，它可以从宿主蛋白中自我移除，并催化其侧翼序列（外链蛋白）与肽键的连接。它们存在于生命的所有三个领域的生物体中，也存在于病毒蛋白中。内切是一个不需要辅助酶或辅因子的翻译后过程。这种自我切除过程被称为蛋白质剪接，类似于RNA内含子从mrna前体剪接。蛋白质剪接只涉及4个分子内反应，以及蛋白内、外链中少量的关键催化残基。蛋白质剪接也可以发生在反式中。在这种情况下，内链被分成N端和c端结构域，它们被合成为单独的组分，每个连接到一个延伸。内部结构域重新组装并将连接的外显子连接成单个功能蛋白。了解顺式和反式蛋白剪接机制导致了内部介导的蛋白质工程应用的发展，如蛋白质纯化、连接、环化和硒蛋白的生产。本文综述了酶联蛋白的催化活性和结构，重点介绍了酶联蛋白在分子生物学和生物技术中的应用。
Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary enzymes or cofactors. This self-excision process is called protein splicing, by analogy to the splicing of RNA introns from pre-mRNA. Protein splicing involves only four intramolecular reactions, and a small number of key catalytic residues in the intein and exteins. Protein-splicing can also occur in trans. In this case, the intein is separated into N- and C-terminal domains, which are synthesized as separate components, each joined to an extein. The intein domains reassemble and link the joined exteins into a single functional protein. Understanding the cis- and trans-protein splicing mechanisms led to the development of intein-mediated protein-engineering applications, such as protein purification, ligation, cyclization, and selenoprotein production. This review summarizes the catalytic activities and structures of inteins, and focuses on the advantages of some recent intein applications in molecular biology and biotechnology.
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影响因子：
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作者：
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DOI：
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发表时间：
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