Controlled Alignment of Multiple Proteins and Nanoparticles with Nanometer Resolution via Backbone-Modified Phosphorothioate DNA and Bifunctional Linkers
Controlled Alignment of Multiple Proteins and Nanoparticles with Nanometer Resolution via Backbone-Modified Phosphorothioate DNA and Bifunctional Linkers
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DOI：
10.1021/ja103739f
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发表时间：
2010-07-07
影响因子：
15
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Lee, Jung Heon;Wong, Ngo Yin;Lu, Yi
链霉亲和素(STV)、肌红蛋白和纳米颗粒通过骨架修饰的硫代磷酸盐DNA和含有生物素和马来酰亚胺的双功能连接物实现了纳米分辨率的可控排列。在三个相邻的PSS中引入三重态生物素修饰显着提高了STV的接合率。通过在双链DNA模板的多个位置放置硫代修饰，形成单体、二聚体和三聚体STV-DNA组件，并将STV放置在受控位置。通过将生物素化的AuNPs结合到STV-DNA复合体上，证明了结合蛋白的活性，这表明该系统作为模板用于形成AuNP二聚体和三聚体，其中STV的间隔为10-30 nm。此外，融化
Controlled alignment of streptavidin (STV), myoglobin, and nanoparticles with nanometer resolution has been achieved via backbone-modified phosphorothioate DNA and biotin- and maleimide-containing bifunctional linkers. Introducing triplet biotin modifications in three adjacent PSs significantly increased the STV conjugation yield. By placing phosphorothioate modifications at multiple positions of a double stranded DNA template, monomer, dimer, and trimer STV-DNA assemblies were formed with the STVs placed at controlled positions. The activity of the conjugated protein has been demonstrated by binding biotinylated AuNPs onto STV-DNA complexes, indicating the use of the system as a template for the formation of AuNP dimers and trimers with STVs separated by distances of 10-30 nm. Furthermore, a melting