Serum from Entamoeba histolytica-infected gerbils selectively suppresses T cell proliferation by inhibiting interleukin-2 production
Serum from Entamoeba histolytica-infected gerbils selectively suppresses T cell proliferation by inhibiting interleukin-2 production
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DOI：
10.1086/314781
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发表时间：
1999-06-01
影响因子：
6.4
通讯作者：
Chadee, K
中科院分区：
文献类型：
--
作者：
Campbell, D;Gaucher, D;Chadee, K
侵袭性阿米巴病中T和B细胞反应的抑制可能是血清介导的。探讨了血清介导的抑制阿米巴肝脓肿沙土鼠脾细胞增殖的机制。与未感染的沙土鼠血清(10%)相比，在感染后第10、30和60天采集的血清样本，而不是在第20天采集的血清样本，同时增强刀豆蛋白A(ConA；T细胞丝裂原)和脂多糖(LPS；B细胞丝裂原)诱导的同种脾细胞的增殖。只有第20天的血清(5%)能抑制未感染沙土鼠脾细胞的增殖，这种抑制机制不依赖于一氧化氮和前列腺素，而与IL-2的产生减少有关。加入外源性IL-2可逆转第20天血清对ConA刺激的细胞增殖的抑制作用。这些结果确定了一种机制，即血清可能有助于在溶组织内阿米巴感染期间瞬时抑制T细胞反应。
Suppression of T and B cell responses during invasive amebiasis may be serum mediated. The mechanism of serum-mediated suppression of spleen cell lymphoproliferation from gerbils with amebic liver abscess was examined. Compared with uninfected gerbil serum (10%), serum samples collected at days 10, 30, and 60, but not at day 20 after infection, augmented both concanavalin A (Con A; T cell mitogen)- and lipopolysaccharide (LPS; B cell mitogen)induced proliferation of homologous spleen cells. Only day 20 serum (>5%) inhibited Con Abut not LPS-induced proliferation of spleen cells from uninfected gerbils, The suppressive mechanism was independent of nitric oxide and prostaglandin but involved reduced interleukin (IL)-2 production. Addition of exogenous IL-2 reversed the suppressive effect of day 20 serum on Con A-stimulated proliferation. These results identify a mechanism whereby serum may contribute to transient suppression of T cell responses during Entamoeba histolytica infections.