Increased expression of macrophage colony-stimulating factor after coronary artery balloon injury is inhibited by intracoronary brachytherapy.
Increased expression of macrophage colony-stimulating factor after coronary artery balloon injury is inhibited by intracoronary brachytherapy.
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冠状动脉球囊损伤后巨噬细胞集落刺激因子表达的增加被冠状动脉内近距离放射治疗所抑制。
DOI：
10.1161/01.cir.0000016048.03020.6c
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发表时间：
2002
期刊：
影响因子：
37.8
通讯作者：
Rajavashisth,TripathiB
中科院分区：
文献类型：
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作者：
Finkelstein,Ariel;Makkar,Raj;Doherty,TerenceM;Vegesna,VijayaR;Tripathi,Pinky;Liu,Ming;Bergman,Jonathan;Fishbein,Michael;Hausleiter,Joerg;Takizawa,Kaname;Rukshin,Vladimir;Shah,PredimanK;Rajavashisth,TripathiB
背景-冠状动脉内近距离放射治疗（ICBT）减少新生内膜增生（NP）的机制尚不清楚。我们假设ICBT通过减少巨噬细胞集落刺激因子（M-CSF）的表达来抑制NP。方法和结果将10只猪的30支冠状动脉分为3组，每组10支：对照组（C）、球囊损伤组（BI）和BI后ICBT组（16戈伊，0.5 mm组织深度，32 P球囊系统）。在24小时（n=3）和7（n=4）和14（n=3）天时处死猪。通过Western blot、ELISA和定量免疫染色评估M-CSF的表达。BI后，在损伤的动脉中观察到M-CSF水平持续增加（至1.4±0.2 nmol/L [ELISA]和染色横截面积的29.4±4.9%[免疫细胞化学];与对照组相比，两者P< 0.001）。与单独BI相比，用ICBT处理BI动脉可降低M-CSF表达（至0.7±0.1 nmol/L [ELISA]和13.5±2.9%染色横截面积[免疫细胞化学];与BI相比P<0.001，与对照相比P =NS），并在14天的研究期间保持与对照M-CSF表达相似。BI后新生内膜厚度增加（4.8±2.9 mm 2;与对照组相比P<0.001），但ICBT使其减少（1.4±0.4 mm 2;与BI相比P<0.001）。结论：在猪冠状动脉中，BI与M-CSF和NP表达增加相关，但ICBT后两者均不发生。ICBT对NP的有益作用涉及抑制M-CSF表达。
Background—The mechanisms underlying the reduced neointimal proliferation (NP) by intracoronary brachytherapy (ICBT) are unknown. We hypothesized that ICBT inhibits NP by reducing expression of macrophage colony–stimulating factor (M-CSF).Methods and Results—Thirty coronary arteries from 10 pigs were divided into 3 groups of 10 each: control (C), balloon injury (BI), and BI followed by ICBT (16 Gy at 0.5-mm tissue depth with a32P balloon system). Pigs were killed at 24 hours (n=3) and at 7 (n=4) and 14 (n=3) days. Expression of M-CSF was assessed by Western blot, ELISA, and quantitative immunostaining. Persistently increased levels of M-CSF after BI (to 1.4±0.2 nmol/L [ELISA] and 29.4±4.9% of cross-sectional area stained [immunocytochemistry];P< 0.001 versus control for both) were observed in the injured arteries. Treatment of BI arteries with ICBT reduced M-CSF expression compared with BI alone (to 0.7±0.1 nmol/L [ELISA] and 13.5±2.9% of cross-sectional area stained [immunocytochemistry];P<0.001 versus BI andP=NS versus control for both) and remained similar to control M-CSF expression for the 14-day study period. Neointimal thickness increased after BI (to 4.8±2.9 mm2;P<0.001 versus control), but this was reduced by ICBT (1.4±0.4 mm2;P<0.001 versus BI).Conclusions—In porcine coronary arteries, BI is associated with increased expression of M-CSF and NP, but neither occurs after ICBT. The beneficial effects of ICBT on NP involve inhibition of M-CSF expression.