The interspecific variations in molecular responses to various doses of heat and cold stress: The case of cereal aphids
The interspecific variations in molecular responses to various doses of heat and cold stress: The case of cereal aphids
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DOI：
10.1016/j.jinsphys.2023.104520
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发表时间：
2023-05-08
影响因子：
2.2
通讯作者：
Colinet，Herve
中科院分区：
文献类型：
--
作者：
Li，Yuan-Jie;Ma，Chun-Sen;Colinet，Herve
由于最近极端温度的频率和幅度增加，昆虫目前受到前所未有的热应激。了解分子对热应激的反应对于理解物种对热应激的反应至关重要。在麦长管蚜协会中发现了三个共生的世界性物种：麦长管蚜、Ropalosiphum padium和Metopolphum dirhoum.早期的报告表明，极端温度的增加通过以不同方式改变种群的增长而导致麦长管蚜协会内优势物种的转移。我们假设，不同物种对压力的不同分子反应可能部分解释了这些变化。热休克蛋白(HSPs)是众所周知的分子伴侣，在抵抗热应激的不利影响方面发挥着重要作用。然而，关于分子伴侣在麦长管蚜中的研究还很少。在这项研究中，我们通过测量中间致死时间(LT50)来比较三种蚜虫的耐热性和耐冷性，并检测了七个基因在相同的热害水平和相同的暴露时间后的表达谱。结果表明，R。Hsp70A是对冷热胁迫反应最强的基因，Hsp70A是对冷热胁迫反应最强的基因，其热诱导基因数量较多，hsp70A、hsp10、hsp60和hsp90mRNA的表达水平显著高于其他两种。DirhoumandS.AvenaWhile的表达在INR中保持不变。帕迪。相比之下，迪氏木霉的耐寒性更强，冷诱导基因也更多。这些结果证实了不同物种在分子胁迫反应上的差异，并表明诱导表达的差异可能与物种的耐热性有关，从而导致相对丰度的变化。
Insects are currently subjected to unprecedented thermal stress due to recent increases in the frequency and amplitude of temperature extremes. Understanding molecular responses to thermal stress is critically important to appreciate how species react to thermal stress. Three co-occurring cosmopolitan species are found within the guild of cereal aphids:Sitobion avenae,Ropalosiphum padiandMetopolophium dirhodum.Earlier reports have shown that increasing frequency of temperature extremes causes a shift in dominant species within guilds of cereal aphids by differently altering the population’s growth. We hypothesize that a differential molecular response to stress among species may partially explain these changes. Heat shock proteins (HSPs) are molecular chaperones well known to play an important role in protecting against the adverse effects of thermal stress. However, few studies on molecular chaperones have been conducted in cereal aphids. In this study, we compared the heat and cold tolerance between three aphid species by measuring the median lethal time (Lt50) and examined the expression profiles of sevenhspgenes after exposures to comparable thermal injury levels and also after same exposure durations. Results showed thatR. padisurvived comparatively better at high temperatures than the two other species but was more cold-sensitive.Hspgenes were induced more strongly by heat than cold stress.Hsp70Awas the most strongly up-regulated gene in response to both heat and cold stress.R. padihad more heat inducible genes and significantly higher mRNA levels ofhsp70A,hsp10,hsp60andhsp90than the other two species.Hspsceased to be expressed at 37 °C inM. dirhodumandS. avenaewhile expression was maintained inR. padi. In contrast,M. dirhodumwas more cold tolerant and had more cold inducible genes than the others. These results confirm species-specific differences in molecular stress responses and suggest that differences in induced expression ofhspsmay be related to species’ thermal tolerance, thus causing the changes in the relative abundance.