A FUCOSE-CONTAINING EPITOPE POTENTIALLY INVOLVED IN GAMETE INTERACTION ON THE HUMAN ZONA-PELLUCIDA
A FUCOSE-CONTAINING EPITOPE POTENTIALLY INVOLVED IN GAMETE INTERACTION ON THE HUMAN ZONA-PELLUCIDA
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DOI：
10.1093/oxfordjournals.humrep.a138744
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发表时间：
1994-08-01
影响因子：
6.1
通讯作者：
BARRIERE, P
中科院分区：
文献类型：
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作者：
LUCAS, H;BERCEGEAY, S;BARRIERE, P
用凝集素和单克隆抗体研究了人、猪和牛透明带的低聚糖部分，这些单克隆抗体特异于含有至少一个末端α - l -焦点的三或四糖表位。从卵泡抽吸中收集动物卵子，从体外受精和胚胎移植项目中收集人类卵子，并将其分为六组。采用直接免疫荧光法对动物或人疫区的凝集素反应性进行检测。金百合凝集素的反应性表明，所研究的三个物种的带中存在α - l聚焦的末端残基。间接免疫荧光法检测到2-25抗体在人畜疫区的反应性，而在动物疫区未发现任何抗体反应性。使用抗Lewis-b血型抗体（2-25），我们观察到该抗原作为人类透明带的固有成分表达，独立于患者的Lewis红细胞表型。抗体2-25在半精区实验中抑制精子带结合，提示该含病灶抗原可能是精子带受体的一部分。
The oligosaccharide moiety of human, porcine and bovine zonae pellucidae was studied with lectins and monoclonal antibodies specific for tri- or tetra-saccharidic epitopes containing at least one terminal alpha-L-fucose. Animal eggs were collected from follicular aspirates, human eggs were collected from in-vitro fertilization and embryo transfer programmes and pooled into six groups. By direct immunofluorescence, the lectins reactivity was detected for the animal or the human zonae pools in the same way. Reactivity of Aleuria aurantia lectin demonstrated the presence of alpha-L-fucose terminal residues in the zonae from the three species studied. By indirect immunofluorescence, the 2-25 antibody reactivity was detected in every pool of human zonae whereas there was no evidence of any antibody reactivity on animal zonae. Using an anti-Lewis-b blood group antibody (2-25), we observed expression of this antigen as an intrinsic component of the human zona pellucida, independently of patients' Lewis red blood cell phenotypes. Antibody 2-25 inhibited the spermatozoa-zona binding in a hemizona assay, suggesting that this fucose-containing antigen could be part of a sperm - zona receptor.