Towards engineering carboxysomes into C3 plants.
Towards engineering carboxysomes into C3 plants.
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DOI：
10.1111/tpj.13139
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发表时间：
2016-07
期刊：
影响因子：
--
通讯作者：
Parry MA
中科院分区：
文献类型：
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作者：
Hanson MR;Lin MT;Carmo-Silva AE;Parry MA
C3植物的光合作用受到碳固定酶Rubisco的限制，Rubisco表现出低周转率，并且可以与O2而不是CO2反应，导致光呼吸。在蓝细菌中，被称为羧基体的细菌微区室通过将CO2集中在Rubisco酶附近来提高光合作用的效率。蓝细菌Rubisco酶比C3植物的Rubisco酶更快，但对CO2的特异性比陆地植物酶低。只有当能够浓缩CO2的微区室也可以安装到叶绿体中时，用具有较低CO2特异性的更快细菌变体取代陆地植物Rubisco才能改善光合作用。我们回顾了目前的信息蓝藻微室和碳浓缩机制，植物转化策略，取代Rubisco在一个模型C3植物与蓝藻Rubisco，并朝着在叶绿体中合成一个羧基体的进展。
Photosynthesis in C3 plants is limited by features of the carbon-fixing enzyme Rubisco, which exhibits a low turnover rate and can react with O2 instead of CO2, leading to photorespiration. In cyanobacteria, bacterial microcompartments known as carboxysomes improve the efficiency of photosynthesis by concentrating CO2 near the enzyme Rubisco. Cyanobacterial Rubisco enzymes are faster than those of C3 plants, though have lower specificity toward CO2 than the land plant enzyme. Replacement of land plant Rubisco by faster bacterial variants with lower CO2 specificity will improve photosynthesis only if a microcompartment capable of concentrating CO2 can also be installed into the chloroplast. We review current information about cyanobacterial microcompartments and carbon-concentrating mechanisms, plant transformation strategies, replacement of Rubisco in a model C3 plant with cyanobacterial Rubisco, and progress toward synthesizing a carboxysome in chloroplasts.