High carbohydrate availability increases LCFA uptake and decreases LCFA oxidation in perfused muscle.
High carbohydrate availability increases LCFA uptake and decreases LCFA oxidation in perfused muscle.
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高碳水化合物利用率可增加灌注肌肉中 LCFA 的吸收并减少 LCFA 氧化。
DOI：
10.1152/ajpendo.00316.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Yee,AliceJ
中科院分区：
文献类型：
--
作者：
Turcotte,LorraineP;Swenberger,JasonR;Yee,AliceJ
为了确定细胞内碳水化合物可用性升高引起的长链脂肪酸 (LCFA) 氧化代谢的变化是否是由于 LCFA 摄取或线粒体转运能力的变化所致，大鼠后躯灌注了 500 μM 棕榈酸酯和 [1-14C] 棕榈酸酯或 [1-14C] 辛酸酯以及低 (LG) 或高 (HG) 碳水化合物可用性。 HG 组的葡萄糖摄取量高于 LG 组（23.6 ± 1.5 vs 4.7 ± 0.9 μmol·g−1·h−1，P< 0.05）。棕榈酸递送量在各组之间没有显着差异，平均为 97.1 ± 4.6 nmol·min−1·g−1（P> 0.05）。 HG（0.125 ± 0.012 和 7.4 ± 1.2 nmol·min−1·g−1）组的棕榈酸酯摄取分数和总值比 LG（0.079 ± 0.009 和 11.8 ± 1.5 nmol·min−1·g−1）组高 60%（P < 0.05）。 HG 组（9.1 ± 1.1% 和 1.31 ± 0.16 nmol·min−1·g−1）的棕榈酸氧化百分比和总棕榈酸值显着低于 LG（23.4 ± 5.2% 和 0.76 ± 0.08 nmol·min−1·g−1）组（P < 0.05）。相反，辛酸的吸收分数和氧化百分比值在各组之间没有显着差异（P>0.05）。丙二酰辅酶A水平与LCFA氧化呈负相关(P<0.05)。这些结果表明，肌肉中碳水化合物的高可用性与 LCFA 氧化的减少有关，这并不是由于 LCFA 摄取的平行减少所致；相反，LCFA 氧化的减少可能是由于丙二酰辅酶 A 抑制线粒体 LCFA 转运所致。
To determine whether changes in long-chain fatty acid (LCFA) oxidative metabolism induced by elevated intracellular carbohydrate availability are due to changes in LCFA uptake or in mitochondrial transport capacity, rat hindquarters were perfused with 500 μM palmitate and [1-14C]palmitate or [1-14C]octanoate as well as with either low (LG) or high (HG) carbohydrate availability. Glucose uptake was higher in the HG vs. LG group (23.6 ± 1.5 vs 4.7 ± 0.9 μmol · g−1· h−1,P< 0.05). Palmitate delivery was not significantly different between groups and averaged 97.1 ± 4.6 nmol · min−1· g−1(P> 0.05). Fractional and total palmitate uptake values were 60% higher (P< 0.05) in the HG (0.125 ± 0.012 and 7.4 ± 1.2 nmol · min−1· g−1) vs. LG (0.079 ± 0.009 and 11.8 ± 1.5 nmol · min−1· g−1) group. Values of percent and total palmitate oxidized were significantly lower (P< 0.05) in the HG (9.1 ± 1.1% and 1.31 ± 0.16 nmol · min−1· g−1) vs. LG (23.4 ± 5.2% and 0.76 ± 0.08 nmol · min−1· g−1) group. Conversely, values of fractional uptake and percent oxidation of octanoate were not significantly different between groups (P> 0.05). Malonyl-CoA levels were inversely correlated with LCFA oxidation (P< 0.05). These results demonstrate that high carbohydrate availability in muscle is associated with a decrease in LCFA oxidation that is not due to a parallel decrease in LCFA uptake; rather, the decrease in LCFA oxidation could be due to malonyl-CoA inhibition of mitochondrial LCFA transport.